Johnston Nicholas, Martini Xavier
Department of Entomology and Nematology, University of Florida, Quincy, FL 32351, USA.
Insects. 2020 Feb 11;11(2):115. doi: 10.3390/insects11020115.
The silverleaf whitefly, , is one of the most destructive agricultural pests in the world, vectoring a large number of devastating viruses, including (TYLCV). When selecting a host, is primarily influenced by a range of visual and olfactory cues. Therefore, elucidating how such cues become modified in the presence of whitefly-vectored begomoviruses is critical to better understanding the epidemiology of many economically important diseases. The goal of this study was to determine how both visual and odor cues interact in the presence of TYLCV. In Y-tube olfactometer assays, whiteflies were submitted to a range of isolated visual and olfactory cues to determine behavioral changes. choices were then compared to both stimuli combined in the presence or absence of TYLCV. Under visual stimuli only, exhibited a visual attraction to the color yellow, TYLCV-infected tomato leaves, and TYLCV-infected tomato volatiles. Attraction was the strongest overall when both visual and olfactory cues from TYLCV-symptomatic tomato plants were combined, as opposed to a single isolated cue. These results highlight the importance of both sensory stimuli during host selection in the presence of an associated begomovirus.
烟粉虱是世界上最具破坏性的农业害虫之一，它能传播大量毁灭性病毒，包括番茄黄化曲叶病毒（TYLCV）。在选择寄主时，烟粉虱主要受一系列视觉和嗅觉线索的影响。因此，阐明在存在烟粉虱传播的双生病毒的情况下这些线索是如何被改变的，对于更好地理解许多经济上重要疾病的流行病学至关重要。本研究的目的是确定在番茄黄化曲叶病毒存在的情况下视觉和气味线索是如何相互作用的。在Y型管嗅觉仪试验中，让烟粉虱接触一系列单独的视觉和嗅觉线索，以确定行为变化。然后将烟粉虱的选择与在有或没有番茄黄化曲叶病毒的情况下两种刺激组合的情况进行比较。仅在视觉刺激下，烟粉虱对黄色、感染番茄黄化曲叶病毒的番茄叶片以及感染番茄黄化曲叶病毒的番茄挥发物表现出视觉吸引力。与单一孤立线索相比，当来自出现番茄黄化曲叶病毒症状的番茄植株的视觉和嗅觉线索组合在一起时，总体吸引力最强。这些结果突出了在存在相关双生病毒的情况下，两种感官刺激在烟粉虱寄主选择过程中的重要性。
