Han Shengwei, Yin Xiteng, Wang Yufeng, Xu Wenguang, Cheng Wei
Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing, China.
Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing, China.
Int J Clin Exp Pathol. 2020 Jan 1;13(1):65-72. eCollection 2020.
This study investigates the prognostic impact of the expression of hypoxia inducible factor (HIF)-1α and Toll-like receptor (TLR) 3 detected by immunohistochemistry in oral squamous cell carcinoma (OSCC). The study also evaluates the treatment outcome by inhibition of the HIF-1α and TLR 3 pathway (nuclear factor [NF]-κB) in an OSCC transplantation model in nude mice. Statistical analysis of immunohistochemical results with clinical findings that included overall survival outcomes was performed for 90 OSCC patients. Forty nude mice were divided into four groups (control; inhibition of HIF-1α; inhibition of NF-κB; and inhibition of HIF-1α and NF-κB). Tumor weight and immunohistochemical results of each group were compared. The results show that co-detection of low HIF-1α/TLR3 expression is significantly correlated with a better prognosis for OSCC patients. Use of an inhibitor of the HIF-1 and TLR3 pathway in an OSCC transplantation model shows a good treatment outcome.
本研究调查了通过免疫组织化学检测的缺氧诱导因子（HIF）-1α和Toll样受体（TLR）3表达在口腔鳞状细胞癌（OSCC）中的预后影响。该研究还在裸鼠OSCC移植模型中通过抑制HIF-1α和TLR 3途径（核因子[NF]-κB）评估治疗效果。对90例OSCC患者的免疫组织化学结果与包括总生存结果在内的临床发现进行了统计分析。40只裸鼠分为四组（对照组；抑制HIF-1α；抑制NF-κB；以及抑制HIF-1α和NF-κB）。比较了每组的肿瘤重量和免疫组织化学结果。结果显示，低HIF-1α/TLR3表达的联合检测与OSCC患者较好的预后显著相关。在OSCC移植模型中使用HIF-1和TLR3途径的抑制剂显示出良好的治疗效果。
