POLA Chemical Industries, Inc., Yokohama, Japan.
Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Skin Res Technol. 2020 Jul;26(4):455-464. doi: 10.1111/srt.12834. Epub 2020 Feb 13.
Lactic acid in the stratum corneum contributes to skin flexibility, making it a useful indicator of skin conditions. It is this useful to devise a simple technique to measure the lactic acid in the stratum corneum. To this aim, a printed chip biosensor was developed to analyze lactic acid in tape stripped (TS) stratum corneum samples.
Lactic acid was extracted from TS stratum corneum samples. A normal lactic acid sensor was prepared by applying lactate oxidase (LOD) to a printed chip. Another lactic acid sensor was prepared using LOD and a mediator osmium polymer immobilized on a printed chip. The amount of lactic acid in the extracted solutions was quantified using either the prepared biosensors or an existing analysis method.
The results measured using the normal lactic acid sensor show low correlation with the results measured using an existing analytical method as a comparison, but those of the mediator osmium lactic acid sensor show high correlation.
The amount of lactic acid in samples extracted from the stratum corneum using a simple TS technique can be simply analyzed with high accuracy using a printed chip biosensor.
角质层中的乳酸有助于皮肤的柔韧性，使其成为皮肤状况的一个有用指标。因此，设计一种简单的技术来测量角质层中的乳酸是很有意义的。为此，开发了一种印刷芯片生物传感器来分析胶带剥离（TS）角质层样品中的乳酸。
从 TS 角质层样品中提取乳酸。通过将乳酸氧化酶（LOD）施加到印刷芯片上，制备了普通乳酸传感器。另一种乳酸传感器是使用 LOD 和固定在印刷芯片上的介体锇聚合物制备的。使用制备的生物传感器或现有的分析方法定量提取溶液中的乳酸含量。
与现有分析方法相比，使用普通乳酸传感器测量的结果相关性较低，但使用介体锇乳酸传感器测量的结果相关性较高。
使用简单的 TS 技术从角质层中提取的样品中的乳酸含量可以使用印刷芯片生物传感器进行简单、准确的分析。
