Department of Chemistry , Université de Montréal , Montréal , Québec H3C 3J7 , Canada.
ACS Sens. 2018 May 25;3(5):929-935. doi: 10.1021/acssensors.7b00871. Epub 2018 May 15.
MicroRNA (miRNA) regulates gene expression and plays a fundamental role in multiple biological processes. However, if both single-stranded RNA and DNA can bind with capture DNA on the sensing surface, selectively amplifying the complementary RNA signal is still challenging for researchers. Fiber-optic surface plasmon resonance (SPR) sensors are small, accurate, and convenient tools for monitoring biological interaction. In this paper, we present a high sensitivity microRNA detection technique using phenylboronic acid functionalized Au nanoparticles (PBA-AuNPs) in fiber-optic SPR sensing systems. Due to the inherent difficulty directly detecting the hybridized RNA on the sensing surface, the PBA-AuNPs were used to selectively amplify the signal of target miRNA. The result shows that the method has high selectivity and sensitivity for miRNA, with a detection limit at 2.7 × 10 M (0.27 pM). This PBA-AuNPs amplification strategy is universally applicable for RNA detection with various sensing technologies, such as surface-enhanced Raman spectroscopy and electrochemistry, among others.
MicroRNA (miRNA) 调控基因表达，在多种生物过程中发挥着基本作用。然而，如果单链 RNA 和 DNA 都可以与传感表面上的捕获 DNA 结合，那么选择性地放大互补 RNA 信号对于研究人员来说仍然具有挑战性。光纤表面等离子体共振 (SPR) 传感器是用于监测生物相互作用的小型、精确和便捷工具。在本文中，我们提出了一种使用苯基硼酸功能化金纳米粒子 (PBA-AuNPs) 的光纤 SPR 传感系统中的高灵敏度 microRNA 检测技术。由于直接检测传感表面上杂交 RNA 的固有困难，因此使用 PBA-AuNPs 选择性地放大靶 miRNA 的信号。结果表明，该方法对 miRNA 具有高选择性和灵敏度，检测限为 2.7×10-12 M（0.27 pM）。这种 PBA-AuNPs 放大策略可普遍适用于各种传感技术（如表面增强拉曼光谱和电化学等）中的 RNA 检测。
