Universidad de Santander, Facultad de Ciencias Exactas, Naturales y Agropecuarias, Grupo de Investigación en Ciencias Agropecuarias-GICA, Bucaramanga, Colombia.
Universidad Industrial de Santander, Facultad de Salud, Grupo de Investigación en Epidemiología Molecular-GIEM, Bucaramanga, Colombia.
Vet Parasitol Reg Stud Reports. 2020 Jan;19:100364. doi: 10.1016/j.vprsr.2019.100364. Epub 2019 Dec 9.
Fasciola hepatica is a parasitic trematode of worldwide distribution, which affects cattle and a large number of mammals, including man. An investigation was carried out to the Colombian Northeastern Mountain between October 2017 and February 2018 with the aim to determine the prevalence and risk factors associated with Fasciola hepatica. Thirty-four farms whose owners agreed to participate in the study were included. Overall, 103 bovines and 97 sheep were examined. A fecal and blood sample was collected from each animal, and all the samples were identified and stored in a refrigerated box to be transported to the Parasitology laboratory for analysis. Fecal samples were processed with a coprological technique, and blood by ELISA test. A written epidemiological survey was applied on all farms. The results indicate 20.5% of coprological prevalence and 41.5% of seroprevalence (SP). Evidence of the parasite was demonstrated in the three municipalities; however, statistical differences were found (P < .05), with higher seropositivity in Duitama municipality (SP = 83.3%). Regarding risk factor, pure breeds of cattle showed 2.5 (SP = 56.1, OR = 2.5) times higher of infection than crossed breed. Cattle and sheep from 12 to 24 months of age, showed 2.5 (SP = 66.7%, OR = 2.5) and 5.9 times (SP = 44.2%, OR = 5.9), respectively, increased infection risk. The presence of snails on the farms had higher odds for testing F. hepatica-positive, therefore, showed to be an important risk factor. The presence of antibodies against F. hepatica in cattle and sheep from the region under study suggests it is endemic and should be controlled by control and prevention programs in the farms.
肝片形吸虫是一种分布广泛的寄生性吸虫，可感染牛和大量哺乳动物，包括人类。2017 年 10 月至 2018 年 2 月，我们对哥伦比亚东北部山区进行了一项调查，旨在确定肝片形吸虫的流行情况和相关危险因素。共有 34 个农场的所有者同意参与研究，总计检查了 103 头牛和 97 只羊。从每只动物采集粪便和血液样本，所有样本均经过鉴定并储存在冷藏箱中，以便运往寄生虫学实验室进行分析。粪便样本采用粪便分析法，血液样本采用 ELISA 检测法。在所有农场都进行了书面流行病学调查。结果表明，粪便分析法的流行率为 20.5%，ELISA 检测法的流行率为 41.5%。虽然在三个城市都发现了该寄生虫，但统计学差异显著（P<0.05），其中杜伊塔马市的阳性率更高（SP=83.3%）。在危险因素方面，纯品种牛的感染风险比杂交品种牛高 2.5 倍（SP=56.1%，OR=2.5）；12-24 月龄的牛和羊的感染风险分别增加 2.5 倍（SP=66.7%，OR=2.5）和 5.9 倍（SP=44.2%，OR=5.9）。农场中蜗牛的存在使检测到肝片形吸虫阳性的几率更高，因此，蜗牛的存在是一个重要的危险因素。该地区牛和羊中存在针对肝片形吸虫的抗体，表明该地区肝片形吸虫病流行，应通过农场的控制和预防计划加以控制。
