Ondruš Jaroslav, Balážová Alena, Baláž Vojtech, Zechmeisterová Kristína, Novobilský Adam, Široký Pavel
CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42, Brno, Czech Republic; Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42, Brno, Czech Republic.
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42, Brno, Czech Republic.
Ticks Tick Borne Dis. 2020 May;11(3):101371. doi: 10.1016/j.ttbdis.2020.101371. Epub 2020 Jan 9.
Candidatus Neoehrlichia mikurensis, the causative agent of tick-borne "neoehrlichiosis" has recently been reported in humans, mammals and ticks in Europe. The aim of this study was to map the distribution of this bacterium in questing ticks in the Czech Republic. A total of 13,325 Ixodes ricinus including 445 larvae, 5270 nymphs and 7610 adults were collected from vegetation by flagging in 140 Czech towns and villages from every region of the Czech Republic. The ticks were pooled into 2665 groups of 5 individuals respecting life stage or sex and tested for the presence of Ca. Neoehrlichia mikurensis by conventional PCR targeting of the groEL gene. The bacterium was detected in 533/2665 pools and 125/140 areas screened, showing an overall estimated prevalence of 4.4 % in ticks of all life stages. Phylogenetic analysis revealed only small genetic diversity among the strains found. Two pools of questing larvae tested positive, suggesting transovarial transmission. According to this study, Ca. Neoehrlichia mikurensis is another tick-borne pathogen widespread in I. ricinus ticks in the Czech Republic.
嗜吞噬细胞无形体(Candidatus Neoehrlichia mikurensis)是蜱传“新埃立克体病”的病原体,最近在欧洲的人类、哺乳动物和蜱类中均有报道。本研究的目的是绘制这种细菌在捷克共和国搜寻蜱类中的分布情况。通过在捷克共和国各个地区的140个城镇和村庄用旗标法从植被中总共采集了13325只蓖麻硬蜱(Ixodes ricinus),其中包括445只幼虫、5270只若虫和7610只成虫。根据蜱的生命阶段或性别,将蜱虫分成2665组,每组5只,通过针对groEL基因的常规PCR检测嗜吞噬细胞无形体的存在情况。在533/2665组和125/140个筛查区域中检测到了这种细菌,显示所有生命阶段蜱虫的总体估计患病率为4.4%。系统发育分析表明,所发现的菌株之间只有很小的遗传多样性。两组搜寻幼虫检测呈阳性,表明存在经卵传播。根据这项研究,嗜吞噬细胞无形体是捷克共和国蓖麻硬蜱中广泛存在的另一种蜱传病原体。