Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovak Republic.
Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovak Republic.
J Biotechnol. 2020 Mar 10;311:1-11. doi: 10.1016/j.jbiotec.2020.02.006. Epub 2020 Feb 11.
Solid-state fermentation is a technique employing microorganisms grown on a solid substrate in the absence of free water. The substrates used in this process are mostly waste from the agro-industry (brans, spent malt grains, distiller grains, etc.) that improves not only the economy of the process but also has positive effect on waste management problems. Zygomycetous fungi are not only able to grow in such conditions but also enrich fermented materials with various types of bioactive compounds. Mucor sp. strains have been identified as producers of gamma-linolenic acid and beta-carotene in submerged fermentation. The aim of the present study was to identify the best microbial producer of gamma-linolenic acid and beta-carotene among four different Mucor strains and to study the requirements for the dual production of these metabolites. Mucor wosnessenskii was identified as the most suitable producer of both metabolites. After optimization of the fermentation conditions, the highest yields obtained were 10.7 g of gamma-linolenic acid/kg of fermented product and 261.5 mg of beta-carotene/kg of fermented product. This yield of beta-carotene is the highest among the results published so far.
固态发酵是一种技术，采用在无游离水的固体基质上生长的微生物。该过程中使用的基质主要是农业工业的废物（麸皮、废麦芽粒、酒糟等），这不仅提高了工艺的经济性，而且对废物管理问题也有积极影响。根霉真菌不仅能够在这样的条件下生长，而且还能使发酵材料富含各种类型的生物活性化合物。米根霉菌株已被鉴定为在液体发酵中产生γ-亚麻酸和β-胡萝卜素的生产者。本研究的目的是从四种不同的根霉菌株中鉴定出生产γ-亚麻酸和β-胡萝卜素的最佳微生物生产者，并研究同时生产这两种代谢产物的要求。沃氏毛霉被鉴定为这两种代谢产物的最合适的生产者。在优化发酵条件后，获得的最高产量分别为 10.7 g/kg 发酵产物的γ-亚麻酸和 261.5 mg/kg 发酵产物的β-胡萝卜素。目前公布的结果中，β-胡萝卜素的这一产量是最高的。
