Nofima, Norwegian Institute of Food, Fisheries and Aquaculture Research, 1433 Ås, Norway.
Molecules. 2020 Feb 12;25(4):792. doi: 10.3390/molecules25040792.
Effective cleaning and disinfection (C&D) is pivotal for the control of in food processing environments. Bacteria in biofilms are protected from biocidal action, and effective strategies for the prevention and removal of biofilms are needed. In this study, different C&D biofilm control strategies on pre-formed biofilms on a conveyor belt material were evaluated and compared to the effect of a conventional chlorinated, alkaline cleaner (agent A). Bacterial reductions up to 1.8 log were obtained in biofilms exposed to daily C&D cycles with normal user concentrations of alkaline, acidic, or enzymatic cleaning agents, followed by disinfection using peracetic acid. No significant differences in bactericidal effects between the treatments were observed. Seven-day-old biofilms were more tolerant to C&D than four-day-old biofilms. Attempts to optimize biofilm eradication protocols for four alkaline, two acidic, and one enzymatic cleaning agent, in accordance with the manufacturers' recommendations, were evaluated. Increased concentrations, the number of subsequent treatments, the exposure times, and the temperatures of the C&D agents provided between 4.0 and >5.5 log reductions in colony forming units (CFU) for seven-day-old biofilms. Enhanced protocols of conventional and enzymatic C&D protocols have the potential for improved biofilm control, although further optimizations and evaluations are needed.
有效的清洁和消毒（C&D）对于控制食品加工环境中的至关重要。生物膜中的细菌免受杀菌作用的影响，因此需要有效的预防和去除生物膜的策略。在这项研究中，评估了不同的 C&D 生物膜控制策略对输送带材料上预先形成的生物膜的影响，并将其与常规氯化、碱性清洁剂（A 剂）的效果进行了比较。在日常 C&D 循环中，使用正常用户浓度的碱性、酸性或酶清洁剂进行清洁，然后使用过氧乙酸进行消毒，可使暴露于其中的生物膜中的细菌减少 1.8 个对数级。在处理方法之间没有观察到杀菌效果的显著差异。与四天龄的生物膜相比，七天龄的生物膜对 C&D 的耐受性更强。根据制造商的建议，尝试优化针对四种碱性、两种酸性和一种酶清洁剂的生物膜清除方案。增加 C&D 试剂的浓度、后续处理的次数、暴露时间和温度，可使七天龄的生物膜的菌落形成单位（CFU）减少 4.0 到 >5.5 个对数级。传统和酶促 C&D 方案的增强方案有可能改善生物膜控制，但需要进一步优化和评估。
