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分离鉴定噬菌体 AbTJ 及其受体结合模块的功能鉴定。

Isolation and Characterization of AbTJ, an Phage, and Functional Identification of Its Receptor-Binding Modules.

机构信息

Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300350, China.

Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China.

出版信息

Viruses. 2020 Feb 12;12(2):205. doi: 10.3390/v12020205.

DOI:10.3390/v12020205
PMID:32059512
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7077233/
Abstract

is an opportunistic pathogen and a major cause of various community-acquired infections Strains of this species can be resistant to multiple antimicrobial agents, leaving limited therapeutic options, also lacking in methods for accurate and prompt diagnosis. In this context, AbTJ, a novel phage that infects MDR-TJ, was isolated and characterized, together with its two tail fiber proteins. Morphological analysis revealed that it belongs to family. Its host range, growth characteristics, stability under various conditions, and genomic sequence, were systematically investigated. Bioinformatic analysis showed that AbTJ consists of a circular, double-stranded 42670-bp DNA molecule which contains 62 putative open reading frames (ORFs). Genome comparison revealed that the phage AbTJ is related to the phage Ab105-1phi (No. KT588074). Tail fiber protein (TFPs) gp52 and gp53 were then identified and confirmed as species-specific proteins. By using a combination of bioluminescent methods and magnetic beads, these TFPs exhibit excellent specificity to detect . The findings of this study can be used to help control opportunistic infections and to provide pathogen-binding modules for further construction of engineered bacteria of diagnosis and treatment.

摘要

是一种机会性病原体，也是多种社区获得性感染的主要原因。该物种的菌株可能对多种抗菌药物具有耐药性，这使得治疗选择有限，并且缺乏准确和及时的诊断方法。在这种情况下，分离并表征了一种新型噬菌体 AbTJ，该噬菌体可感染 MDR-TJ 及其两种尾部纤维蛋白。形态分析表明，它属于 科。系统研究了其宿主范围、生长特性、各种条件下的稳定性以及基因组序列。生物信息学分析表明，AbTJ 由一个圆形、双链的 42670-bp DNA 分子组成，其中包含 62 个推定的开放阅读框 (ORF)。基因组比较表明，噬菌体 AbTJ 与噬菌体 Ab105-1phi（No. KT588074）有关。然后鉴定并确认尾部纤维蛋白 (TFPs) gp52 和 gp53 为种特异性蛋白。通过组合使用生物发光方法和磁珠，这些 TFPs 表现出优异的特异性，可用于检测 。本研究的发现可用于帮助控制机会性感染，并为进一步构建用于诊断和治疗的工程菌提供病原体结合模块。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/485f/7077233/e8258906928c/viruses-12-00205-g015.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/485f/7077233/1c0e6f5a16e0/viruses-12-00205-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/485f/7077233/2d26d25746da/viruses-12-00205-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/485f/7077233/ab7619bd2f4c/viruses-12-00205-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/485f/7077233/d3d8f351e91a/viruses-12-00205-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/485f/7077233/29e3d6090833/viruses-12-00205-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/485f/7077233/e8258906928c/viruses-12-00205-g015.jpg

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