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一种用于无瓣伊蚊(双翅目)的长短读长高质量基因组组装方法

A High-Quality Genome Assembly from Short and Long Reads for the Non-biting Midge (Diptera).

机构信息

Molecular Ecology Group, Senckenberg Biodiversity and Climate Research Centre, Frankfurt am Main, Hessen, Germany.

Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, CA.

出版信息

G3 (Bethesda). 2020 Apr 9;10(4):1151-1157. doi: 10.1534/g3.119.400710.

Abstract

is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species. Making use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make ' genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species, and the respective recombination rate among the first insect recombination rates at all. The genome assembly and associated resources will be highly valuable to the broad community working with dipterans in general and chironomids in particular. The estimated recombination rate will help evolutionary biologists gaining a better understanding of commonalities and differences of genomic patterns in insects.

摘要

是生态毒理学、分子遗传学、发育生物学和生态学等多个领域的重要研究物种。然而,迄今为止,仅有一个碎片化的草图基因组存在,这阻碍了该物种近期大量群体基因组研究的开展。我们利用 50 个 NGS 数据集,通过短读长和长读长数据,提出了一个混合基因组组装,使基因组成为已发表的最连续的双翅目基因组之一,这也是该物种的第一个完整的线粒体基因组,以及所有昆虫重组率中第一个公布的重组率。基因组组装和相关资源将对一般双翅目、特别是摇蚊科的广大研究群体具有重要价值。估计的重组率将帮助进化生物学家更好地理解昆虫基因组模式的共性和差异。

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