Research and Development Centre, Regional Specialist Hospital, Kamienskiego 73a, 51-154, Wroclaw, Poland.
Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
Arch Immunol Ther Exp (Warsz). 2020 Feb 14;68(1):5. doi: 10.1007/s00005-020-00571-9.
Adipose tissue is a reliable source of mesenchymal stromal cells (MSC) for use in regenerative medicine. The aim of this pilot study was to describe the method, and assess the safety and the potential efficacy of transplantation of autologous adipose tissue-derived MSC for the treatment of chronic venous stasis ulcers. Study group consisted of 11 patients (mean age: 66.6 ± 9.5 years) with chronic venous stasis ulcers. Adipose tissue was harvested by tumescent-aspiration method. Stromal cells were separated using a dedicated closed system in a real-time bedside manner. The phenotype of cells was determined immediately after separation. Cell concentrate was implanted subcutaneously around the wound and the wound bed. All ulcers were assessed planimetrically before autotransplantation and every two weeks during the six-month follow-up. During the study all patients received standard local and general treatment. The preparation contained an average of 5.6 × 10 ± 4 × 10 cells per milliliter. The phenotype of 65-82% of transplanted cells expressed MSC markers: CD73 CD90 and CD34. An improvement was observed in 75% of ulcers. The data showed highly significant negative correlation (p < 0.0001) between wound size and wound closure degree. There was no correlation of ulcer healing with other parameters evaluated, including age of the patients. No serious side effects were observed. Autotransplantation of adipose tissue stromal cells may be a safe and promising treatment method for chronic venous ulcers.
脂肪组织是间充质基质细胞（MSC）的可靠来源，可用于再生医学。本初步研究的目的是描述该方法，并评估自体脂肪组织源性 MSC 移植治疗慢性静脉淤滞性溃疡的安全性和潜在疗效。研究组包括 11 名（平均年龄：66.6±9.5 岁）慢性静脉淤滞性溃疡患者。采用肿胀抽吸法采集脂肪组织。使用专用的封闭系统在实时床边条件下分离基质细胞。分离后立即确定细胞表型。细胞浓缩物被皮下植入伤口周围和伤口床。在自体移植前和 6 个月的随访期间每两周对所有溃疡进行平面评估。在研究期间，所有患者均接受标准的局部和全身治疗。该制剂每毫升平均含有 5.6×10±4×10 个细胞。移植细胞中 65-82%的表型表达 MSC 标志物：CD73、CD90 和 CD34。75%的溃疡得到改善。数据显示，伤口大小与伤口闭合程度之间存在高度显著的负相关（p<0.0001）。溃疡愈合与其他评估参数（包括患者年龄）之间没有相关性。未观察到严重的副作用。自体脂肪组织基质细胞移植可能是治疗慢性静脉溃疡的一种安全且有前途的方法。
