Breen-Norris James O, Siow Bernard, Walsh Claire, Hipwell Ben, Hill Ioana, Roberts Thomas, Hall Matt G, Lythgoe Mark F, Ianus Andrada, Alexander Daniel C, Walker-Samuel Simon
Centre for Advanced Biomedical Imaging, Division of Medicine, University College London, London, UK.
Microstructure Imaging Group, Centre for Medical Imaging Computing, University College London, London, UK.
Magn Reson Med. 2020 Sep;84(3):1543-1551. doi: 10.1002/mrm.28207. Epub 2020 Feb 14.
To combine numerical simulations, in vitro and in vivo experiments to evaluate the feasibility of measuring diffusion exchange across the cell membrane with diffusion exchange spectroscopy (DEXSY).
DEXSY acquisitions were simulated over a range of permeabilities in nerve tissue and yeast substrates. In vitro measurements were performed in a yeast substrate and in vivo measurements in mouse tumor xenograft models, all at 9.4 T.
Diffusion exchange was observed in simulations over a physiologically relevant range of cell permeability values. In vitro and in vivo measures also provided evidence of diffusion exchange, which was quantified with the Diffusion Exchange Index (DEI).
Our findings provide preliminary evidence that DEXSY can be used to make in vivo measurements of diffusion exchange and cell membrane permeability.
结合数值模拟、体外和体内实验，以评估采用扩散交换光谱法（DEXSY）测量跨细胞膜扩散交换的可行性。
在神经组织和酵母底物的一系列渗透率范围内模拟DEXSY采集。在酵母底物中进行体外测量，并在小鼠肿瘤异种移植模型中进行体内测量，均在9.4 T条件下进行。
在生理相关的细胞渗透率值范围内的模拟中观察到扩散交换。体外和体内测量也提供了扩散交换的证据，并用扩散交换指数（DEI）进行了量化。
我们的研究结果提供了初步证据，表明DEXSY可用于体内测量扩散交换和细胞膜渗透率。
