Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Biochim Biophys Acta Gen Subj. 2020 Jun;1864(6):129558. doi: 10.1016/j.bbagen.2020.129558. Epub 2020 Feb 13.
Cutaneous leishmaniasis is a parasitic disease, which is difficult to treat due to high drug resistance and adverse side effects. Photodynamic therapy by ultraviolet radiation using materials with high photocatalytic features like titanium dioxide nanoparticles (TiO-NPs) is an emerging treatment for this disease. In this study, TiO-NPs with ultraviolet (UV) radiation were administered as photodynamic therapy against Leishmania Major (LM) promastigotes.
Two forms of TiO viz. including Anatase and Rutile were administered in two UV ranges< UVA and UVB for different time periods (30 and 60 min). Finally, 24 and 48 h after incubation, the MTS test was performed and cell survival percentage was calculated.
The mean size of Anatase and Rutile-NPs is approximately 32.5 and 50.9 nm respectively by DLS and FE-SEM, and crystal phase is emphasized by XRD. The combined treatment of LM with TiO-NPs and UV has significant effects on LM promastigotes, which vary depending on NP and UV types. The synergistic effect was anticipated in the groups irradiated by UV-B in the presence of Rutile NPs.
The combined treatment with UV- radiation and TiO-NPs can be effective in killing the promastigotes of Leishmania major. The proper concentration of NPs and the type of UV-radiation must be taken into consideration. The results suggest improved treatment methods, after proper in vivo studies.
皮肤利什曼病是一种寄生虫病，由于耐药性高和不良反应，治疗困难。利用具有高光催化特性的材料（如二氧化钛纳米粒子（TiO-NPs））进行的紫外辐射光动力疗法是治疗这种疾病的一种新兴方法。在这项研究中，用 TiO-NPs 和紫外线（UV）辐射对利什曼原虫（LM）前鞭毛体进行光动力治疗。
两种形式的 TiO，包括锐钛矿和金红石，在两个不同的紫外光范围<UVA 和 UVB 下分别照射不同的时间（30 和 60 分钟）。最后，在孵育 24 和 48 小时后，进行 MTS 测试并计算细胞存活率。
DLS 和 FE-SEM 显示锐钛矿和金红石-NPs 的平均粒径分别约为 32.5 和 50.9nm，XRD 则强调了晶体相。LM 与 TiO-NPs 和 UV 的联合治疗对 LM 前鞭毛体有显著影响，其效果因 NP 和 UV 类型而异。在存在金红石 NPs 的情况下，UV-B 照射的各组预计会产生协同作用。
UV 辐射与 TiO-NPs 的联合治疗可有效杀死利什曼原虫前鞭毛体。必须考虑 NPs 的适当浓度和 UV 辐射类型。在进行适当的体内研究后，这些结果表明可以改进治疗方法。
