Institute for Lasers, Photonics and Biophotonics, Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY, USA.
Institute for Lasers, Photonics and Biophotonics, Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY, USA; Department of Anesthesiology, University at Buffalo, The State University of New York, Buffalo, NY, USA; Department of Medicine, Division of Allergy, Immunology, and Rheumatology, State University of New York at Buffalo, Clinical Translational Research Center, Buffalo, NY, USA.
Nanomedicine. 2020 Apr;25:102172. doi: 10.1016/j.nano.2020.102172. Epub 2020 Feb 13.
We introduce the use of laser ablation to develop a multi-drug encapsulating theranostic nanoformulation for HIV-1 antiretroviral therapy. Laser ablated nanoformulations of ritonavir, atazanavir, and curcumin, a natural product that has both optical imaging and pharmacologic properties, were produced in an aqueous media containing Pluronic® F127. Cellular uptake was confirmed with the curcumin fluorescence signal localized in the cytoplasm. Formulations produced with F127 had improved water dispersibility, are ultrasmall in size (20-25 nm), exhibit enhanced cellular uptake in microglia, improve blood-brain barrier (BBB) crossing in an in vitro BBB model, and reduce viral p24 by 36 fold compared to formulations made without F127. This work demonstrates that these ultrasmall femtosecond laser-ablated nanoparticles are effective in delivering drugs across the BBB for brain therapy and show promise as an effective method to formulate nanoparticles for brain theranostics, reducing the need for organic solvents during preparation.
我们介绍了使用激光烧蚀来开发一种多药物包封的治疗性纳米制剂，用于 HIV-1 抗逆转录病毒治疗。在含有普朗尼克 F127 的水介质中，激光烧蚀了利托那韦、阿扎那韦和姜黄素（一种具有光学成像和药理特性的天然产物）的纳米制剂。用姜黄素的荧光信号在细胞质中定位来确认细胞摄取。用 F127 生产的制剂具有更好的水分散性，尺寸非常小（20-25nm），在小神经胶质细胞中表现出增强的细胞摄取，在体外 BBB 模型中提高血脑屏障（BBB）的穿透性，与没有 F127 的制剂相比，降低病毒 p24 达 36 倍。这项工作表明，这些超小的飞秒激光烧蚀纳米粒子在递送到大脑治疗的 BBB 中是有效的，并为脑治疗学中的纳米粒子制剂提供了一种有前途的方法，减少了在制备过程中对有机溶剂的需求。
