CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Department of Biology, University of Minho, Braga, Portugal; Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Tras-os-Montes e Alto Douro, Vila Real, Portugal.
Food Chem. 2020 Jun 30;316:126298. doi: 10.1016/j.foodchem.2020.126298. Epub 2020 Jan 27.
Vine Pruning residue was submitted to conventional heating and ohmic heating (OH) for the extraction of bioactive compounds and analyzed for total phenolic content (TPC), polyphenolic profile, antioxidant activity, antimicrobial activity and anticancer activity. The OH extracts were obtained using Low electric field (496.0 V/cm) or Intermediate electric field - IEF (840.0 V/cm). The tests were performed using 45% (v/v) ethanol-water extraction solution at 80 °C at different extraction times (20-90 min). The extract that stood out among the others concerning anticancer potential was the one obtained by OH when used, IEF, where the TPC was significantly higher than in the other extracts which correlated with higher antioxidant, antimicrobial and anti-proliferative activity on different tumor cell lines (HepG2, MDA-MB-231, MCF-7 and Caco2). Vine pruning OH extracts obtained using green solvents by an eco-friendly procedure were revealed as a source of compounds with relevant antioxidant and anticancer activity.
葡萄修剪残余物经常规加热和欧姆加热（OH）处理，用于提取生物活性化合物，并分析总酚含量（TPC）、多酚谱、抗氧化活性、抗菌活性和抗癌活性。OH 提取物采用低电场（496.0 V/cm）或中间电场 - IEF（840.0 V/cm）获得。测试使用 45%（v/v）乙醇-水提取溶液在 80°C 下进行不同的提取时间（20-90 分钟）。在抗癌潜力方面，OH 提取的提取物比其他提取物更为突出，IEF 条件下 TPC 显著高于其他提取物，这与不同肿瘤细胞系（HepG2、MDA-MB-231、MCF-7 和 Caco2）的更高抗氧化、抗菌和抗增殖活性相关。通过环保工艺用绿色溶剂获得的葡萄修剪 OH 提取物被证明是具有相关抗氧化和抗癌活性的化合物的来源。
