Department of Botany, University of Kalyani, Nadia, India.
Agricultural and Ecological Research Unit, Indian Statistical Institute, Kolkata, India.
Int J Phytoremediation. 2020;22(10):1048-1058. doi: 10.1080/15226514.2020.1725871. Epub 2020 Feb 16.
Arsenic contamination in agricultural soil now imposing a major threat to crop productivity and causing several hazardous health effects through percolation in food chain. Bioremediation, an efficient way of soil health restoration toward sustainability offered by some soil-borne microorganisms, has been reported. The present work deals with application of two potent arsenic-tolerant bacterial strains ( A01 and B05), obtained from natural sources in modulating overall growth and antioxidant defense against arsenic-treated rice plants. Between the two, former could reduce arsenic uptake up to 56% (roots) and 85% (shoots), and the preceding one up to 31% (roots) and 65% (shoots) in a hydroponic environment. Germination percentage was noted to be enhanced significantly ( ≤ 0.05). Expression of oxidative stress defensive enzymes such as superoxide dismutase, peroxidase and catalase have been augmented at seedling stages (21 days) toward detoxification of arsenic imposed excess ROS generation. Increment of leaf Thiobarbituric acid reactive substances due to arsenic exposure have been ameliorated by both the bacterial application. Phenolic and flavonoid mediated free radical scavenging ability of the test plants elevated significantly ( ≤ 0.05). The present work revealed that, selected bacterial strains can perform efficient bioremediation against arsenic pollutant rice cultivation.
土壤中的砷污染现已对作物生产力构成重大威胁，并通过食物链在食物中渗透造成多种危险的健康影响。据报道，一些土壤传播的微生物提供了一种有效的土壤健康恢复方法，即生物修复，以实现可持续性。本工作涉及应用两种从天然来源获得的具有很强耐砷性的细菌菌株（A01 和 B05），来调节整体生长和对抗砷处理的水稻植物的抗氧化防御。在这两种菌株中，前者可将砷的吸收量降低多达 56%（根部）和 85%（茎部），而后者则可降低多达 31%（根部）和 65%（茎部）。在水培环境中。发芽率显著提高（≤0.05）。在幼苗阶段（21 天），表达的氧化应激防御酶，如超氧化物歧化酶、过氧化物酶和过氧化氢酶，增强了，以解毒过量的 ROS 产生的砷。由于砷暴露，叶片中硫代巴比妥酸反应物质的增加通过两种细菌的应用得到了改善。测试植物中酚类和类黄酮介导的自由基清除能力显著提高（≤0.05）。本工作表明，所选的细菌菌株可以有效地对砷污染的水稻种植进行生物修复。
