Institute of Process engineering in Life Science 2: Technical Biology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
School of Molecular & Cell Biology, Faculty of Science, University of the Witwatersrand, WITS 2050, Johannesburg, South Africa.
Sci Rep. 2020 Feb 17;10(1):2780. doi: 10.1038/s41598-020-59672-2.
Trichosporonaceae incorporates six genera of physiologically and ecologically diverse fungi including both human pathogenic taxa as well as yeasts of biotechnological interest, especially those oleagenic taxa that accumulate large amounts of single cell oils (SCOs). Here, we have undertaken comparative genomic analysis of thirty-three members of the family with a view to gain insight into the molecular determinants underlying their lifestyles and niche specializations. Phylogenomic analysis revealed potential misidentification of three strains which could impact subsequent analyses. Evaluation of the predicted proteins coding sequences showed that the free-living members of the family harbour greater numbers of carbohydrate active enzymes (CAZYmes), metallo- and serine peptidases compared to their host-associated counterparts. Phylogenies of selected lipid biosynthetic enzymes encoded in the genomes of the studied strains revealed disparate evolutionary histories for some proteins inconsistent with the core genome phylogeny. However, the documented oleagenic members distinctly cluster based on the constitution of the upstream regulatory regions of genes encoding acetyl-CoA carboxylase (ACC), ATP-citrate synthase (ACS) and isocitrate dehydrogenase [NADP] (ICDH), which are among the major proteins in the lipid biosynthetic pathway of these yeasts, suggesting a possible pattern in the regulation of these genes.
Trichosporonaceae 包含六个生理和生态多样化的真菌属，包括人类病原真菌以及具有生物技术应用价值的酵母，特别是那些能够积累大量单细胞油脂（SCO）的产油真菌。在这里，我们对该科的三十三个成员进行了比较基因组分析，以期深入了解其生活方式和生态位特化的分子决定因素。系统发育基因组分析揭示了三种菌株可能存在潜在的错误鉴定，这可能会影响后续的分析。对预测蛋白编码序列的评估表明，与宿主相关的菌株相比，自由生活的菌株具有更多的碳水化合物活性酶（CAZYmes）、金属和丝氨酸肽酶。对所研究菌株基因组中编码脂质生物合成酶的选择蛋白的系统发育分析表明，一些蛋白质的进化历史与核心基因组系统发育不一致，与核心基因组系统发育不一致。然而，所记录的产油成员根据编码乙酰辅酶 A 羧化酶（ACC）、三羧酸循环酶（ACS）和异柠檬酸脱氢酶 [NADP]（ICDH）的基因上游调控区的组成明显聚类，这些基因是这些酵母中脂质生物合成途径的主要蛋白之一，这表明这些基因的调控可能存在一种模式。
