用益生元绿香蕉抗性淀粉和益生菌凝结芽孢杆菌孢子进行合生元补充可改善炎症性肠病小鼠模型中的肠道炎症。

Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.

作者信息

Shinde Tanvi, Perera Agampodi Promoda, Vemuri Ravichandra, Gondalia Shakuntla V, Beale David J, Karpe Avinash V, Shastri Sonia, Basheer Waheedha, Southam Benjamin, Eri Rajaraman, Stanley Roger

机构信息

Centre for Food Safety and Innovation, Tasmanian Institute of Agriculture, University of Tasmania, Launceston, TAS, 7250, Australia.

School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, TAS, 7250, Australia.

出版信息

Eur J Nutr. 2020 Dec;59(8):3669-3689. doi: 10.1007/s00394-020-02200-9. Epub 2020 Feb 17.

PURPOSE

The research goal is to develop dietary strategies to help address the growing incidence of inflammatory bowel diseases (IBD). This study has investigated the effectiveness of green banana resistant starch (GBRS) and probiotic Bacillus coagulans MTCC5856 spores for the amelioration of dextran-sulfate sodium (DSS)-induced colitis in mice.

METHODS

Eight-week-old C57BL/6 mice were fed standard rodent chow diet supplemented with either B. coagulans, GBRS or its synbiotic combination. After 7 days supplementation, colitis was induced by adding 2% DSS in drinking water for 7 days while continuing the supplemented diets. Animal health was monitored and after 14 days all animals were sacrificed to measure the biochemical and histochemical changes associated with each supplement type.

RESULTS

The disease activity index and histological damage score for DSS-control mice (6.1, 17.1, respectively) were significantly higher (p < 0.0001) than the healthy mice. Synbiotic supplementation alleviated these markers (- 67%, - 94% respectively) more adequately than B. coagulans (- 52%, - 58% respectively) or GBRS (- 57%, - 26%, respectively) alone. Compared to DSS-control synbiotic supplementation significantly (p < 0.0001) maintained expressions of tight junction proteins. Moreover, synbiotic effects accounted for ~ 40% suppression of IL-1β and ~ 29% increase in IL-10 levels in serum while also reducing C-reactive protein (- 37%) compared to that of the DSS-control. While, B. coagulans alone could not induce additional levels of short-chain fatty acid (SCFA) production beyond the caecum, the synbiotic combination with GBRS resulted in substantial increased SCFA levels across the whole length of the colon.

CONCLUSION

The synbiotic supplementation with B. coagulans and GBRS ameliorated the overall inflammatory status of the experimental IBD model via synergistic functioning. This supports researching its application in mitigating inflammation in human IBD.

目的

本研究的目标是制定饮食策略，以帮助应对炎症性肠病（IBD）发病率不断上升的问题。本研究调查了绿香蕉抗性淀粉（GBRS）和益生菌凝结芽孢杆菌MTCC5856孢子对改善葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的有效性。

方法

给8周龄的C57BL/6小鼠喂食补充了凝结芽孢杆菌、GBRS或其合生元组合的标准啮齿动物饲料。补充7天后，通过在饮用水中添加2% DSS持续7天来诱导结肠炎，同时继续喂食补充饲料。监测动物健康状况，14天后处死所有动物，以测量与每种补充剂类型相关的生化和组织化学变化。

结果

DSS对照组小鼠的疾病活动指数和组织学损伤评分（分别为6.1和17.1）显著高于健康小鼠（p < 0.0001）。合生元补充剂比单独使用凝结芽孢杆菌（分别为-52%，-58%）或GBRS（分别为-57%，-26%）更能充分缓解这些指标（分别为-67%，-94%）。与DSS对照组相比，合生元补充剂显著（p < 　0.0001）维持紧密连接蛋白的表达。此外，与DSS对照组相比，合生元的作用导致血清中IL-1β水平抑制约40%，IL-10水平升高约29%，同时还降低了C反应蛋白（-37%）。虽然，单独的凝结芽孢杆菌在盲肠之外不能诱导额外水平的短链脂肪酸（SCFA）产生，但与GBRS的合生元组合导致整个结肠长度的SCFA水平大幅增加。