School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, Tasmania 7250, Australia.
Centre for Food Safety and Innovation, Tasmanian Institute of Agriculture, University of Tasmania, Launceston, Tasmania 7250, Australia.
Nutrients. 2018 Sep 6;10(9):1255. doi: 10.3390/nu10091255.
Recent evidence suggests that gut microbiota shifts can alter host metabolism even during healthy aging. DDS-1, a probiotic strain, has shown promising probiotic character in vitro, as well as in clinical studies. The present study was carried out to investigate whether DDS-1 can modulate the host metabolic phenotype under the condition of age-affected gut microbial shifts in young and aging C57BL/6J mice. Collected fecal samples were analyzed using 16S rRNA gene sequencing for identifying gut microbiota and untargeted gas chromatography-mass spectrometry (GC-MS) metabolomics analysis. Gut microbial shifts were observed in the control groups (young and aging), leading to an alteration in metabolism. Principal coordinate analysis (PCoA) of microbiota indicated distinct separation in both the DDS-1-treated groups. DDS-1 increased the relative abundances of beneficial bacteria, such as and spp., and reduced the relative levels of opportunistic bacteria such as spp. Metabolic pathway analysis identified 10 key pathways involving amino acid metabolism, protein synthesis and metabolism, carbohydrate metabolism, and butanoate metabolism. These findings suggest that modulation of gut microbiota by DDS-1 results in improvement of metabolic phenotype in the aging mice.
最近的证据表明，肠道微生物群的变化即使在健康衰老期间也可以改变宿主的新陈代谢。DDS-1 是一种益生菌菌株，在体外和临床研究中都表现出有前途的益生菌特性。本研究旨在研究 DDS-1 是否可以在年轻和衰老的 C57BL/6J 小鼠的年龄相关肠道微生物群变化的情况下调节宿主的代谢表型。通过 16S rRNA 基因测序收集粪便样本进行分析，以鉴定肠道微生物群和非靶向气相色谱-质谱（GC-MS）代谢组学分析。在对照组（年轻和衰老）中观察到肠道微生物群的变化，导致代谢发生改变。微生物群落的主坐标分析（PCoA）表明 DDS-1 处理组的分离明显。DDS-1 增加了有益细菌的相对丰度，如 和 ，并降低了机会性细菌的相对水平，如 。代谢途径分析确定了涉及氨基酸代谢、蛋白质合成和代谢、碳水化合物代谢和丁酸盐代谢的 10 个关键途径。这些发现表明，DDS-1 对肠道微生物群的调节可改善衰老小鼠的代谢表型。
