Wang Lina, Wang Chaokui, Wu Tong, Sun Fengyuan
Tianjin First Central Hospital, Tianjin, China.
Tianjin Medical University Eye Hospital, Tianjin, China.
J Cell Commun Signal. 2020 Jun;14(2):193-205. doi: 10.1007/s12079-020-00550-x. Epub 2020 Feb 18.
Retinoblastoma (RB) is one of the most common ophthalmic tumors, and most of the patients have been identified as advanced at the time of diagnosis, which is directly related to high mortality. Recent studies showed that long noncoding RNA (lncRNA) and miRNAs play key roles in the development、progression、or treatment of cancer, such as RB. However, the role of lncRNA -TP73-AS1 in RB remains unclear. In this study, we performed functional and mechanistic investigation of miRNA-874-3p-TP73-AS1 interaction in RB. The experiments results revealed that miRNA-874-3p had anti-oncogenic functions in RB. Moreover, the bioinformatics analysis shown that TP73-AS1 could bind to miRNA-874-3p. TP73-AS1 was inversely correlated with miRNA-874-3p expression. Furthermore, studies confirmed that TP73-AS1 negatively regulated miRNA-874-3p expression via functioning as a ceRNA. In a word, our results suggest that the TP73-AS1/ miRNA-874-3p / TFAP2B (transcription factor activating enhancer-binding protein 2B) pathway contributes to the progression of RB, which may provide novel insights into the function of lncRNA-driven retinoblastogenesis. Graphical abstract.
视网膜母细胞瘤（RB）是最常见的眼科肿瘤之一，大多数患者在诊断时已被确定为晚期，这与高死亡率直接相关。最近的研究表明，长链非编码RNA（lncRNA）和微小RNA（miRNA）在癌症（如RB）的发生、发展或治疗中起关键作用。然而，lncRNA -TP73-AS1在RB中的作用仍不清楚。在本研究中，我们对RB中miRNA-874-3p-TP73-AS1的相互作用进行了功能和机制研究。实验结果表明，miRNA-874-3p在RB中具有抗癌功能。此外，生物信息学分析显示TP73-AS1可以与miRNA-874-3p结合。TP73-AS1与miRNA-874-3p的表达呈负相关。此外，研究证实TP73-AS1通过作为竞争性内源RNA（ceRNA）负向调节miRNA-874-3p的表达。总之，我们的结果表明TP73-AS1/miRNA-874-3p/转录因子激活增强子结合蛋白2B（TFAP2B）途径促进了RB的进展，这可能为lncRNA驱动的视网膜母细胞瘤发生的功能提供新的见解。图形摘要。
