Bao Chenhui, Guo Lin
Department of General surgery, ShengJing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang City, Liaoning Province, China 110004.
J Cancer. 2022 Feb 7;13(4):1324-1335. doi: 10.7150/jca.66683. eCollection 2022.
Gastric cancer (GC) is a common gastrointestinal malignancy. Evidence suggests that long non-coding RNAs (lncRNAs) influence mRNA expression to induce GC progression. We aim to investigate the function and regulatory mechanism of TP73-AS1 in GC. We detected TP73-AS1, miR-27b-3p, and TMED5 (Transmembrane P24 Trafficking Protein 5) by real-time polymerase chain reaction (RT-PCR). Similarly, the protein levels of TMED5 and wnt/β-catenin were detected by western-blot. The colony formation and Cell-Counting Kit-8 (CCK-8) assay detected cell proliferation. Transwell and scrape assay tested cell migration and invasion. Dual-luciferase reporter assays confirmed directed binding targets. Tumor xenograft in nude mice checked the result . TP73-AS1 over-expressed in GC. Suppressed TP73-AS1 inhibited cell proliferation, migration, and invasion. However, down-regulated miR-27b-3p could reverse the effects of weakenTP73-AS1 on the progression of GC. Moreover, TMED5 was also up-regulated in GC. Both TP73-AS1 and TMED5 were the direct target of miR-27b-3p. Meanwhile, miR-27b-3p was a negative correlation with TP73-AS1 and TMED5. The TP73-AS1/miR-27b-3p/TMED5 axis regulate wnt/β-catenin pathway. TP73-AS1 promoted GC proliferation, migration, and invasion by sponging miR-27b-3p to regulate TMED5. TP73-AS1 was a potential onco-lncRNA in GC.
胃癌（GC）是一种常见的胃肠道恶性肿瘤。有证据表明，长链非编码RNA（lncRNAs）会影响mRNA表达，从而促使胃癌进展。我们旨在研究TP73-AS1在胃癌中的功能及调控机制。我们通过实时聚合酶链反应（RT-PCR）检测TP73-AS1、miR-27b-3p和跨膜P24转运蛋白5（TMED5）。同样，通过蛋白质免疫印迹法检测TMED5和wnt/β-连环蛋白的蛋白水平。集落形成实验和细胞计数试剂盒-8（CCK-8）实验检测细胞增殖情况。Transwell实验和划痕实验检测细胞迁移和侵袭能力。双荧光素酶报告基因实验确定直接的结合靶点。通过裸鼠肿瘤异种移植实验验证结果。TP73-AS1在胃癌中过表达。抑制TP73-AS1可抑制细胞增殖、迁移和侵袭。然而，下调miR-27b-3p可逆转减弱的TP73-AS1对胃癌进展的影响。此外，TMED5在胃癌中也上调。TP73-AS1和TMED5均是miR-27b-3p的直接靶点。同时，miR-27b-3p与TP73-AS1和TMED5呈负相关。TP73-AS1/miR-27b-3p/TMED5轴调节wnt/β-连环蛋白通路。TP73-AS1通过海绵吸附miR-27b-3p来调节TMED5，从而促进胃癌的增殖、迁移和侵袭。TP73-AS1是胃癌中一种潜在的致癌lncRNA。
