Department of Obstetrics and Gynecology, School of Medicine, Firat University, 23119 Elazig, Turkey.
Department of Internal Medicine (Endocrinology and Metabolism Diseases), School of Medicine, Firat University, 23119 Elazig, Turkey.
Peptides. 2020 Apr;126:170277. doi: 10.1016/j.peptides.2020.170277. Epub 2020 Feb 14.
Subfatin and spexin are two novel adipokines implicated in glucose homeostasis. This study was designed to investigate changes in blood subfatin and spexin levels during gestational diabetes mellitus (GDM) and childbirth, and define the mechanisms of these hormones in the physiopathology of GDM. A total of 60 pregnant women, comprising 30 diagnosed with GDM and 30 with normal gestation, were included in the study. The diagnosis of GDM was made through a 75-g oral glucose tolerance test (OGTT) administered between 24 and 28 weeks of pregnancy. The amounts of subfatin, spexin, and insulin were measured in blood samples by enzyme-linked immunosorbent assays; lipid profiles, glucose, and other biochemical parameters were measured by using an autoanalyzer. Levels of subfatin and spexin were significantly higher in blood samples drawn at baseline (before OGTT) in mothers with GDM compared to those with normal gestation. Similar observations were made in maternal and cord blood sampled at the end of pregnancy. However, at delivery, the increase in subfatin and spexin concentrations observed at baseline was abrogated in both groups of pregnant women, although levels in mothers with GDM were comparatively higher. These results show that levels of subfatin and spexin increased because of GDM and suggest that these hormones could be potential biomarkers for the diagnosis and management of GDM.
脂肪因子 subfatin 和 spexin 与葡萄糖稳态有关，本研究旨在探讨妊娠期糖尿病（GDM）及分娩过程中血液 subfatin 和 spexin 水平的变化，并明确这些激素在 GDM 病理生理学中的作用机制。共纳入 60 例孕妇，30 例为 GDM，30 例为正常妊娠。GDM 的诊断采用 75g 口服葡萄糖耐量试验（OGTT），于妊娠 24-28 周进行。通过酶联免疫吸附试验（ELISA）检测血液样本中的 subfatin、spexin 和胰岛素含量，采用自动分析仪检测血脂谱、血糖和其他生化参数。与正常妊娠孕妇相比，GDM 孕妇基线（OGTT 前）时血液样本中的 subfatin 和 spexin 水平明显升高，在妊娠末期时，孕妇和脐带血中的这两种物质也观察到同样的升高。然而，在分娩时，两组孕妇的 subfatin 和 spexin 浓度均较基线水平升高，但 GDM 孕妇的水平相对较高。这些结果表明，subfatin 和 spexin 水平升高与 GDM 有关，提示这些激素可能是 GDM 诊断和管理的潜在生物标志物。
