National Demonstration Center for Experimental Biology Education, Northeast Normal University, Changchun, 130024, PR China.
National Demonstration Center for Experimental Biology Education, Northeast Normal University, Changchun, 130024, PR China.
Carbohydr Polym. 2020 Apr 15;234:115936. doi: 10.1016/j.carbpol.2020.115936. Epub 2020 Jan 30.
An antioxidant-active polysaccharide (WMP) with a molecular weight of 26.91 kDa was isolated from Mentha haplocalyx Briq by water extraction, ethanol precipitation and gel filtration. According to HPLC, methylation, GC-MS, and 1D/2D nuclear magnetic resonance spectroscopy, WMP is a heteropolysaccharide composed of Gal (84.2 %), Glc (9.8 %), Man (2.8 %) and Ara (3.2 %) with (1→6)-α-d-Galp and (1→4, 6)-α-d-Galp residues in the backbone and (1→6)-α-d-Galp and (1→6)-α-d-Glcp residues in the branch. The branch point was located at C-4 of (1→4, 6)-α-d-Galp residue with a branching degree of 19.71 %. WMP showed remarkably high scavenging ability for 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals, ferrous ion chelating activity and ferric reducing powder in vitro. In vivo result showed that WMP oral administration substantially increased the activities of antioxidant enzymes, including SOD, GSH-Px and CAT, and decreased MDA levels in the serum and liver of d-Gal-induced aging mice. Therefore, WMP can be an effective natural antioxidant.
从薄荷中用水提醇沉和凝胶过滤法提取出一种抗氧化活性多糖（WMP），分子量为 26.91kDa。根据 HPLC、甲基化、GC-MS 和 1D/2D 核磁共振波谱分析，WMP 是一种杂多糖，由 Gal（84.2%）、Glc（9.8%）、Man（2.8%）和 Ara（3.2%）组成，主链由（1→6）-α-d-Galp 和（1→4,6）-α-d-Galp 残基组成，支链由（1→6）-α-d-Galp 和（1→6）-α-d-Glcp 残基组成。支链的分支点位于（1→4,6）-α-d-Galp 残基的 C-4 位，分支度为 19.71%。WMP 对 1,1-二苯基-2-苦基肼（DPPH）和羟基自由基具有显著的清除能力，具有体外亚铁离子螯合活性和铁还原粉末活性。体内结果表明，WMP 口服给药可显著提高抗氧化酶（包括 SOD、GSH-Px 和 CAT）的活性，降低 D-半乳糖诱导衰老小鼠血清和肝脏中 MDA 水平。因此，WMP 可以作为一种有效的天然抗氧化剂。
