School of Pharmaceutlcal Sciences, Changchun University of Chinese Medicine, Changchun 130024, China.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
Carbohydr Polym. 2018 Sep 15;196:246-253. doi: 10.1016/j.carbpol.2018.05.037. Epub 2018 May 17.
The structure and antioxidant activity of a neutral polysaccharide (named as EFPN-1) from Epimedium koreanum Nakai were studied. Based on the results from high performance gel permeation chromatography, methylation GC-MS and 1D/2D nuclear magnetic resonance spectroscopy, EFPN-1 was a branched polysaccharide with a molecular weight of 21.9 kD and containing over 60% of glucose. Its backbone was composed of (1 → 4)-β-d-Glcp and (1 → 3)-β-d-Galp, branched partially at O-6 of both residues, with a degree of branching of 40.4% and terminated mainly with Ara and Gal, as well as trace amount of Gal. Antioxidant tests showed that EFPN-1 exhibited a strong capacity on DPPH and hydroxyl radical scavenging, ferrous ion chelation and reducing antioxidant powder. EFPN-1 has potential to be developed as a natural antioxidant.
从朝鲜淫羊藿中提取的中性多糖（命名为 EFPN-1）的结构和抗氧化活性研究。根据高效凝胶渗透色谱、甲基化 GC-MS 和 1D/2D 核磁共振波谱的结果，EFPN-1 是一种支链多糖，分子量为 21.9 kD，含有超过 60%的葡萄糖。其主链由（1→4）-β-d-Glcp 和（1→3）-β-d-Galp 组成，部分在两个残基的 O-6 处支化，支化度为 40.4%，主要以阿拉伯糖和半乳糖为末端，还有少量的半乳糖。抗氧化试验表明，EFPN-1 对 DPPH 和羟基自由基清除、亚铁离子螯合和还原抗氧化粉均具有较强的能力。EFPN-1 有潜力开发为天然抗氧化剂。
