2010 - 2018年泰国急性胃肠炎儿科患者中札如病毒的基因重组与多样性
Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010-2018.
作者信息
Kumthip Kattareeya, Khamrin Pattara, Ushijima Hiroshi, Chen Limin, Li Shilin, Maneekarn Niwat
机构信息
Department of Microbiology, Chiang Mai University, Faculty of Medicine, Chiang Mai, Thailand.
Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand.
出版信息
PeerJ. 2020 Feb 6;8:e8520. doi: 10.7717/peerj.8520. eCollection 2020.
BACKGROUND
Human sapovirus (SaV) is an etiologic agent of acute gastroenteritis (AGE) in all age groups worldwide. Genetic recombination of SaV has been reported from many countries. So far, none of SaV recombinant strain has been reported from Thailand. This study examined the genetic recombination and genotype diversity of SaV in children hospitalized with AGE in Chiang Mai, Thailand.
METHODS
Stool samples were collected from children suffering from diarrhea who admitted to the hospitals in Chiang Mai, Thailand between 2010 and 2018. SaV was detected by RT-PCR and the polymerase and capsid gene sequences were analysed.
RESULTS
From a total of 3,057 samples tested, 50 (1.6%) were positive for SaV. Among positive samples, SaV genotype GI.1 was the most predominant genotype (40%; 20/50), followed by GII.1 and GII.5 (each of 16%; 8/50), GI.2 (14%; 7/50), GIV.1 (4%; 2/50), and GI.5 (2%; 1/50). In addition, 4 SaV recombinant strains of GII.1/GII.4 were identified in this study (8%; 4/50).
CONCLUSIONS
The data revealed the genetic diversity of SaV circulating in children with AGE in Chiang Mai, Thailand during 2010 to 2018 and the intragenogroup SaV recombinant strains were reported for the first time in Thailand.
背景
人札幌病毒（SaV）是全球所有年龄组急性胃肠炎（AGE）的病原体。许多国家都报道了SaV的基因重组情况。到目前为止，泰国尚未报道过SaV重组毒株。本研究调查了泰国清迈患有AGE的住院儿童中SaV的基因重组和基因型多样性。
方法
收集了2010年至2018年间在泰国清迈医院住院的腹泻儿童的粪便样本。通过逆转录聚合酶链反应（RT-PCR）检测SaV，并分析聚合酶和衣壳基因序列。
结果
在总共检测的3057份样本中，50份（1.6%）SaV呈阳性。在阳性样本中，SaV基因型GI.1是最主要的基因型（40%；20/50），其次是GII.1和GII.5（各占16%；8/50），GI.2（14%；7/50），GIV.1（4%；2/50）和GI.5（2%；1/50）。此外，本研究中鉴定出4株GII.1/GII.4的SaV重组毒株（8%；4/50）。
结论
数据揭示了2010年至2018年期间泰国清迈患有AGE的儿童中循环的SaV的基因多样性，并首次在泰国报道了组内SaV重组毒株。
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