基于胍基杯[5]芳烃与荧光素报告基团对的维生素B6磷酸酯超分子串联分析

Yue Yu-Xin, Kong Yong, Yang Fan, Zheng Zhe, Hu Xin-Yue, Guo Dong-Sheng

College of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Key Laboratory of Functional Polymer Materials (Ministry of Education), Tianjin Key Laboratory of Biosensing and Molecular Recognition Nankai University Tianjin 300071 P. R. China.

Research Institute of Petroleum Engineering, Sinopec Beijing 100101 P. R. China.

ChemistryOpen. 2019 Dec 13;8(12):1437-1440. doi: 10.1002/open.201900316. eCollection 2019 Dec.

Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal-5'-phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of ALP). This supramolecular tandem assay system offers an opportunity to monitor the activity of ALP in a label-free, continuous, and real-time manner. More importantly, a calibration curve can be given for selective and quantitative detection of pyridoxal-5'-phosphate (biomarker for several diseases).

已选择胍基杯[5]芳烃和荧光素报告基团对，基于对碱性磷酸酶（ALP）的底物磷酸吡哆醛、ALP的产物吡哆醛以及ALP的产物磷酸盐的差异识别，建立一种超分子串联分析原理。这种超分子串联分析系统提供了一个以无标记、连续和实时方式监测ALP活性的机会。更重要的是，可以给出一条校准曲线，用于选择性和定量检测磷酸吡哆醛（几种疾病的生物标志物）。