Zheng Zhe, Geng Wen-Chao, Gao Jie, Wang Yu-Ying, Sun Hongwei, Guo Dong-Sheng
College of Chemistry , State Key Laboratory of Elemento-Organic Chemistry , Key Laboratory of Functional Polymer Materials , Ministry of Education , Nankai University , Tianjin 300071 , China . Email:
Collaborative Innovation Center of Chemical Science and Engineering , Nankai University , Tianjin 300071 , China.
Chem Sci. 2018 Jan 9;9(8):2087-2091. doi: 10.1039/c7sc04989g. eCollection 2018 Feb 28.
We designed a water-soluble guanidinium-modified calix[5]arene to target lysophosphatidic acid (LPA), an ideal biomarker for early diagnosis of ovarian and other gynecologic cancers, achieving binding on the nanomolar level. An indicator displacement assay, coupled with differential sensing, enabled ultrasensitive and specific detection of LPA. Moreover, we show that using a calibration line, the LPA concentration in untreated serum can be quantified in the biologically relevant low μM range with a detection limit of 1.7 μM. The reported approach is feasible for diagnosing ovarian and other gynecologic cancers, particularly at their early stages.
我们设计了一种水溶性胍基修饰杯[5]芳烃，用于靶向溶血磷脂酸（LPA），LPA是卵巢癌和其他妇科癌症早期诊断的理想生物标志物，该修饰杯芳烃能在纳摩尔水平上实现结合。一种指示剂置换分析方法，结合差异传感，能够对LPA进行超灵敏且特异的检测。此外，我们表明，使用校准曲线，可以在生物学相关的低微摩尔范围内对未处理血清中的LPA浓度进行定量，检测限为1.7微摩尔。所报道的方法对于诊断卵巢癌和其他妇科癌症是可行的，尤其是在癌症早期阶段。
