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无刺蜂(蜜蜂科,无刺蜂亚科)蜂王幼虫的首个从头转录组组装数据集。

The first dataset of de novo transcriptome assembly of (Apidae, Meliponinae) queen larva.

作者信息

Tamizi Amin Asyraf, Nazaruddin Nazrul Hisham, Yeong Wee Chien, Mohd Radzi Muhammad Faris, Jaafar Mohd Azwan, Sekeli Rogayah

机构信息

Agri-Omics and Bioinformatics Programme, Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), 43400 Serdang, Selangor, Malaysia.

出版信息

Data Brief. 2020 Feb 1;29:105235. doi: 10.1016/j.dib.2020.105235. eCollection 2020 Apr.

DOI:10.1016/j.dib.2020.105235
PMID:32071998
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7013129/
Abstract

is a species of stingless bee recently domesticated (or reared) for honey production in a few Southeast Asian countries namely Malaysia and Indonesia. Being categorized in the clade Corbiculata together with the honeybees ( spp.) and bumble bees ( spp.), the stingless bees are highly social in which the colony members are subjected to labor division where a queen functions as the reproductive caste. In this data article, we provide a resource encompassing a transcriptome profile (de novo assembled) from queen larva - the first report of transcriptome assembly for this species. The generated data is pivotal for the characterization of important genes and biological pathways in order to further improve our understanding on the developmental biology, behavior, social structure and ecological needs of this eusocial hymenopteran insect from the molecular aspect. The raw RNA sequencing data is available at NCBI Sequence Read Archive (SAR) under the accession number SRP230250 and the assembled reads are deposited at DDBJ/EMBL/Genbank as Transcriptome Shotgun Assembly (TSA) under the accession GIIH00000000.

摘要

是一种无刺蜂,最近在马来西亚和印度尼西亚等一些东南亚国家被驯化(或饲养)用于蜂蜜生产。与蜜蜂(属)和熊蜂(属)一起被归类在Corbiculata进化枝中,无刺蜂具有高度社会性,群体成员存在劳动分工,其中蜂后作为生殖阶层。在这篇数据文章中,我们提供了一份资源,包括来自[蜂种名称]蜂后幼虫的转录组图谱(从头组装)——这是该物种转录组组装的首次报告。所生成的数据对于重要基因和生物学途径的表征至关重要,以便从分子层面进一步提高我们对这种群居膜翅目昆虫的发育生物学、行为、社会结构和生态需求的理解。原始RNA测序数据可在NCBI序列读取存档库(SRA)中获取,登录号为SRP230250,组装后的 reads 作为转录组鸟枪法组装(TSA)存于DDBJ/EMBL/Genbank,登录号为GIIH00000000。 (注:原文中“a transcriptome profile (de novo assembled) from queen larva”这里“from”后面缺少具体蜂种名称,翻译时根据语境补充为“来自[蜂种名称]蜂后幼虫”)

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c6/7013129/76670c0da901/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c6/7013129/76670c0da901/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c6/7013129/76670c0da901/gr1.jpg

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本文引用的文献

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Methyl farnesoate epoxidase (mfe) gene expression and juvenile hormone titers in the life cycle of a highly eusocial stingless bee, Melipona scutellaris.法尼醇甲酯环氧酶(mfe)基因表达与高度社会性无刺蜂小盾蜜蜂生命周期中的保幼激素滴度
J Insect Physiol. 2017 Aug;101:185-194. doi: 10.1016/j.jinsphys.2017.08.001. Epub 2017 Aug 9.
2
Corset: enabling differential gene expression analysis for de novo assembled transcriptomes.Corset:为从头组装的转录组实现差异基因表达分析。
Genome Biol. 2014 Jul 26;15(7):410. doi: 10.1186/s13059-014-0410-6.
3
Full-length transcriptome assembly from RNA-Seq data without a reference genome.
无参考基因组的 RNA-Seq 数据的全长转录组组装。
Nat Biotechnol. 2011 May 15;29(7):644-52. doi: 10.1038/nbt.1883.