Feng Zhen, Zhang Minghu, Liu Xin, Liang Dongyu, Liu Xiaojuan, Hao Ming, Liu Dengcai, Ning Shunzong, Yuan Zhongwei, Jiang Bo, Chen Xuejiao, Chen Xue, Zhang Lianquan
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
J Appl Genet. 2020 Sep;61(3):313-322. doi: 10.1007/s13353-020-00555-7. Epub 2020 Apr 5.
Triticum boeoticum (2n = 2x = 14, AA) contains beneficial traits for common wheat improvement. The discrimination of A-genome chromosomes from A-genome chromosomes is an important step in gene transfer from T. boeoticum to common wheat. In this study, fluorescence in situ hybridization (FISH) analysis using nine oligonucleotide probes revealed high divergence between chromosomes of the common wheat germplasm Crocus and T. boeoticum accession G52. The combination of Oligo-pTa535-HM and Oligo-pSc119.2-HM can differentiate A and A chromosomes within homologous groups 2, 4, 5, and 6; chromosomes 2A and 6A can be identified by using (ACT), (CTT), and (GAA). The probes Oligo-pTa713 and (ACT) can be utilized for the identification of chromosomes 1A and 3A, respectively. Probes (CAG) and (CAC) can be applied in the identification of 7A. Moreover, probe combinations consisting of Oligo-pTa535-HM and (AAC) with (ACT) or (CTT) and of Oligo-pTa535-HM and Oligo-pTa713 with (CAC) or (CTT) will help discriminate the A-genome chromosomes of T. boeoticum. These probes are being used as potential markers to select common wheat Crocus-T. boeoticum G52 alien chromosome lines. Moreover, FISH patterns are highly divergent between A- and A-genome chromosomes, indicating that obvious chromosome structural variations arose during wheat evolution.
野生一粒小麦（2n = 2x = 14，AA）含有有助于改良普通小麦的有益性状。区分A基因组染色体与A基因组染色体是将野生一粒小麦的基因转移到普通小麦中的重要一步。在本研究中，使用9种寡核苷酸探针进行的荧光原位杂交（FISH）分析显示，普通小麦种质藏红花和野生一粒小麦种质G52的染色体之间存在高度差异。寡核苷酸-pTa535-HM和寡核苷酸-pSc119.2-HM的组合可以区分同源群2、4、5和6内的A和A染色体；染色体2A和6A可以通过使用（ACT）、（CTT）和（GAA）来鉴定。探针寡核苷酸-pTa713和（ACT）可分别用于鉴定染色体1A和3A。探针（CAG）和（CAC）可用于鉴定7A。此外，由寡核苷酸-pTa535-HM和（AAC）与（ACT）或（CTT）组成的探针组合，以及由寡核苷酸-pTa535-HM和寡核苷酸-pTa713与（CAC）或（CTT）组成的探针组合，将有助于区分野生一粒小麦的A基因组染色体。这些探针正被用作选择普通小麦藏红花-野生一粒小麦G52异源染色体系的潜在标记。此外，A基因组和A基因组染色体之间的FISH模式高度不同，表明在小麦进化过程中出现了明显的染色体结构变异。
