Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan, 31538, Republic of Korea.
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju, Wonju, 26493, Republic of Korea.
Anaerobe. 2019 Jun;57:99-106. doi: 10.1016/j.anaerobe.2019.04.001. Epub 2019 Apr 4.
Enterotoxigenic Bacteroides fragilis (ETBF) can form biofilms in the colon mucosal membrane and may promote chronic infection and tumor formation. The objective of this study was to determine the effect of zerumbone extracted from Zingiber zerumbet (L.) Smith, on B. fragilis biofilm formation. Inhibitory effects of zerumbone on planktonic cell growth and biofilm formation were examined by crystal-violet biofilm assays and XTT metabolic assays. Results showed that zerumbone significantly inhibited biofilm formation and eradicated established biofilms. Furthermore, B. fragilis biofilms inhibited by zerumbone were observed by confocal laser scanning microscopy. qRT-PCR was used to support the phenotypic results and to investigate the expression levels of an efflux pump-related gene (bmeB). Specifically, among the efflux pump-related genes, zerumbone significantly suppressed the expression level of bmeB12. These results indicate that zerumbone might be a promising candidate as an anti-biofilm and antimicrobial agent to treat and prevent biofilm-related infections caused by B. fragilis.
产肠毒素脆弱拟杆菌（ETBF）可以在结肠黏膜形成生物膜，并可能促进慢性感染和肿瘤形成。本研究旨在确定从姜科植物盾叶姜（Zingiber zerumbet（L.）Smith）中提取的莪术醇对脆弱拟杆菌生物膜形成的影响。通过结晶紫生物膜测定法和 XTT 代谢测定法，研究了莪术醇对浮游细胞生长和生物膜形成的抑制作用。结果表明，莪术醇能显著抑制生物膜的形成并清除已形成的生物膜。此外，通过共聚焦激光扫描显微镜观察到被莪术醇抑制的脆弱拟杆菌生物膜。qRT-PCR 用于支持表型结果，并研究与外排泵相关的基因（bmeB）的表达水平。具体而言，在与外排泵相关的基因中，莪术醇显著抑制了 bmeB12 的表达水平。这些结果表明，莪术醇可能是一种有前途的候选药物，可作为一种抗生物膜和抗菌剂，用于治疗和预防由脆弱拟杆菌引起的与生物膜相关的感染。
