临床样本中皮肤利什曼病的监测：2018年巴西阿马帕州圭亚那利什曼原虫的分布情况
Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amapá, Brazil, 2018.
作者信息
Almeida Ariely Nunes Ferreira de, Nascimento Luciana de Cássia Silva do, Sousa Edith Silvia Moura de Moura, Oliveira Afonso José Diger de, Sena Maria Gorete de, Resende Breno Maués de, Chaves Raimunda Cleide Gonçalves, Garcez Lourdes Maria
机构信息
Universidade Federal do Amapá, Departamento de Ciências Biológicas e da Saúde, Macapá, AP, Brasil.
Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua, PA, Brasil.
出版信息
Epidemiol Serv Saude. 2020 Feb 17;29(1):e2018504. doi: 10.5123/S1679-49742020000100007. eCollection 2020.
OBJECTIVE
to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amapá State, Brazilian Amazon.
METHODS
this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amapá; individuals with CL receiving care from January-May/2018 were recruited; clinical data and skin biopsies were obtained; from extracted DNA (phenol-chloroform) we amplified the hsp70-234 gene region (PCR) for nucleotide sequencing (Applied Biosystems: ABI3500XL).
RESULTS
38 individuals were interviewed, examined and diagnosed; men predominated (28/38; mean age=32.5±11.3); lesions (most ulcers: 37/38) measuring 0,4-10mm (34/38) and ≥11mm (4/38) were multiple in 20/38 individuals; diagnosis of L. braziliensis (1), L. naiffi (1), L. infantum (1), L. (Viannia) sp. (1), L. amazonensis (2) and L. guyanensis (32); individuals infected with L. guyanensis (32/38) lived in 9/10 municipalities represented in the sample, and 17/32 of these had multiple lesions.
CONCLUSION
presence of Leishmania guyanensis predominated and was frequently associated with multiple lesions.
目的
调查巴西亚马逊州阿马帕州一系列本地皮肤利什曼病（CL）病例中的利什曼原虫种类。
方法
这是一项描述性生态学研究，于2018年1月至10月在阿马帕州的CL诊断参考中心进行；招募了2018年1月至5月接受治疗的CL患者；获取临床数据和皮肤活检样本；从提取的DNA（酚-氯仿法）中扩增hsp70-234基因区域（PCR）用于核苷酸测序（应用生物系统公司：ABI3500XL）。
结果
对38人进行了访谈、检查和诊断；男性居多（28/38；平均年龄=32.5±11.3岁）；病变（大多数为溃疡：37/38）大小为0.4 - 10mm（34/38）和≥11mm（4/38），20/38的个体有多处病变；诊断出巴西利什曼原虫（1例）、奈菲利什曼原虫（1例）、婴儿利什曼原虫（1例）、（维安尼亚利什曼原虫）属（1例）、亚马逊利什曼原虫（2例）和圭亚那利什曼原虫（32例）；感染圭亚那利什曼原虫的个体（32/38）生活在样本所代表的10个市中的9个，其中17/32有多处病变。
结论
圭亚那利什曼原虫占主导地位，且常与多处病变相关。
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