Zhou J H, Xing D, Ma H M, Zhao Y, Zhao Y H, Wei H Q
Department of Otorhinolaryngology, the First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2020 Feb 7;55(2):154-158. doi: 10.3760/cma.j.issn.1673-0860.2020.02.013.
To observe the effect of olfactory training on mice with olfactory dysfunction induced by 3-methylindole (3-MI). Thirty-one male BALB/c mice were randomly divided into 3 groups by random digits table: control group (group A, 10), olfactory dysfunction group (group B, 10) and olfactory dysfunction+olfactory training group (group C, 11). Mice in group B and group C were intraperitoneally injected with 150 mg/kg 3-MI to induce olfactory dysfunction model, while mice in group A were intraperitoneally injected with corn oil of the same volume. From the first day after injection, mice in group C were treated with 4 kinds of odors by inhalation, while mice in group B were treated with distilled water by inhalation, with 2 times/d, 30 min/time/kind of odor, and continuous training for 28 d. Group A was not treated. Buried food pellet tests were conducted before injection and at 7, 14, 21 and 28 days after injection, respectively. The olfactory epithelium was harvested for observation of the number of olfactory marker protein (OMP) and the thickness of olfactory epithelium on the 28th day after injection. SPSS 23.0 software was used for statistical analysis. Before injection, all mice in each group had no olfactory dysfunction. At the 7th, 14th, 21st and 28th days after injection, the food finding time of mice in group C was shorter than that in group B, and the difference was statistically significant ((175.88±100.50) s (266.73±46.83) s, (132.00±84.62) s (264.10±48.50) s, (103.57±77.43) s (197.43±69.78) s, (67.79±32.54) s (176.63±61.06) s, all 0.05), but food finding time of mice in group B and C was longer than that in group A (the food finding time of group A at the 7th, 14th, 21st and 28th days after injection was (27.13±5.36) s, (25.83±7.28) s, (23.13±2.72) s, (26.63±7.60) s, respectively, all 0.05). At the 28th day after olfactory training, the number of OMP positive cells in group B and C were fewer than that in group A, and the difference was statistically significant ((108.00±28.19)/HP (288.22±84.06)/HP, (199.33±58.55)/HP (288.22±84.06)/HP, all 0.05). The number of OMP positive cells in group C were higher than that in group B (0.05). The number of OMP positive cells had negative correlation with food finding time (=-0.886, 0.01). As for the thickness of the olfactory epithelium, the thickness of group B was thinner than that in group A and C, and the difference was statistically significant ((59.57±31.27) μm (114.55±40.70)μm (90.54±37.72) μm, all 0.05). Olfactory training can accelerate the recovery of olfactory function in 3-MI-induced olfactory impaired mice.
观察嗅觉训练对3-甲基吲哚（3-MI）诱导的嗅觉功能障碍小鼠的影响。将31只雄性BALB/c小鼠通过随机数字表随机分为3组：对照组（A组，10只）、嗅觉功能障碍组（B组，10只）和嗅觉功能障碍+嗅觉训练组（C组，11只）。B组和C组小鼠腹腔注射150 mg/kg 3-MI以诱导嗅觉功能障碍模型，而A组小鼠腹腔注射相同体积的玉米油。从注射后第一天起，C组小鼠通过吸入4种气味进行处理，而B组小鼠通过吸入蒸馏水进行处理，每天2次，每种气味每次30分钟，持续训练28天。A组不进行处理。分别在注射前以及注射后7、14、21和28天进行埋藏食物颗粒试验。在注射后第28天采集嗅上皮，观察嗅标记蛋白（OMP）的数量和嗅上皮的厚度。使用SPSS 23.0软件进行统计分析。注射前，每组所有小鼠均无嗅觉功能障碍。在注射后第7、14、21和28天，C组小鼠寻找食物的时间短于B组，差异具有统计学意义（（175.88±100.50）秒对（266.73±46.83）秒，（132.00±84.62）秒对（264.10±48.50）秒，（103.57±77.43）秒对（197.43±69.78）秒，（67.79±32.54）秒对（176.63±61.06）秒，均P<0.05），但B组和C组小鼠寻找食物的时间长于A组（A组在注射后第7、14、21和28天寻找食物的时间分别为（27.13±5.36）秒、（25.83±7.28）秒、（23.13±2.72）秒、（26.63±7.60）秒，均P<0.05）。嗅觉训练后第28天，B组和C组OMP阳性细胞数量少于A组，差异具有统计学意义（（108.00±28.19）/高倍视野对（288.22±84.06）/高倍视野，（199.33±58.55）/高倍视野对（288.22±84.06）/高倍视野，均P<0.05）。C组OMP阳性细胞数量高于B组（P<0.05）。OMP阳性细胞数量与寻找食物时间呈负相关（r=-0.886，P<0.01）。至于嗅上皮厚度，B组厚度比A组和C组薄，差异具有统计学意义（（59.57±31.27）μm对（114.55±40.70）μm对（90.54±37.72）μm，均P<0.05）。嗅觉训练可加速3-MI诱导的嗅觉受损小鼠嗅觉功能的恢复。
