Couto Joana, Figueirinha Artur, Batista Maria Teresa, Paranhos António, Nunes Carla, Gonçalves Lídia Maria, Marto Joana, Fitas Manuel, Pinto Pedro, Ribeiro Helena Margarida, Pina Maria Eugénia
Faculty of Pharmacy of University of Coimbra, University of Coimbra, 3000-548 Coimbra, Portugal.
LAQV, REQUIMTE, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal.
Antioxidants (Basel). 2020 Feb 14;9(2):154. doi: 10.3390/antiox9020154.
L. (), popularly known as wild strawberry, is a plant from the Rosaceae family, found in temperate and subtropical areas of the northern hemisphere. leaves have been shown to have antiseptic, emollient, and dermatological protection properties, due to the presence of bioactive compounds, such as flavonoids, phenolic acids, ellagitannins, and proanthocyanidins. In this study, a extract was obtained by an optimized extraction process, and was characterized by HPLC, ROS scavenging activity, cytotoxicity assays in HaCaT cells, and tyrosinase inhibitory activity determination. The most active extract was then incorporated in a hydrogel with hydroxyethylcellulose at 2% (), which was characterized at the physicochemical, stability, cytotoxicity, and ROS scavenging activity levels to evaluate its quality, safety, and efficacy. In vivo studies, human repeat insult patch testing, and an assay to determine their antioxidant efficacy, were also performed. The results showed that the extracts had antioxidant activity and that the extract-based hydrogel exhibited cutaneous compatibility, acceptability and antioxidant efficacy, being stable, and suitable for topical application.
L.（俗称野草莓）是蔷薇科植物，生长于北半球的温带和亚热带地区。由于含有生物活性化合物，如黄酮类化合物、酚酸、鞣花单宁和原花青素，其叶子已被证明具有防腐、润肤和皮肤保护特性。在本研究中，通过优化提取工艺获得了一种提取物，并通过高效液相色谱、活性氧清除活性、HaCaT细胞的细胞毒性测定以及酪氨酸酶抑制活性测定对其进行了表征。然后将活性最高的提取物与2%的羟乙基纤维素混合制成水凝胶，对其进行了物理化学、稳定性、细胞毒性和活性氧清除活性水平的表征，以评估其质量、安全性和有效性。还进行了体内研究、人体重复刺激斑贴试验以及测定其抗氧化功效的试验。结果表明该提取物具有抗氧化活性，且基于该提取物的水凝胶表现出皮肤相容性、可接受性和抗氧化功效，稳定且适合局部应用。
