Foo Rui Qing, Jahromi Mohammad Faseleh, Chen Wei Li, Ahmad Syahida, Lai Kok Song, Idrus Zulkifli, Liang Juan Boo
Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia.
Arianabiotech co. No 118, Parsian Industrial Zone, Mashad 9354195366, Khorasan Razavi, Iran.
Microorganisms. 2020 Feb 14;8(2):255. doi: 10.3390/microorganisms8020255.
serovar (ser.) Enteritidis ( Enteritidis) is a foodborne pathogen often associated with contaminated poultry products. This study evaluated the anti-adherence and intracellular clearance capability of oligosaccharides extracted from palm kernel cake (PKC), a by-product of the palm oil industry, and compared its efficacy with commercial prebiotics- fructooligosaccharide (FOS) and mannanoligosaccharide (MOS)-against Enteritidis in vitro. Based on the degree of polymerization (DP), PKC oligosaccharides were further divided into 'Small' (DP ≤ 6) and 'Big' (DP > 6) fractions. Results showed that the Small and Big PKC fractions were able to reduce ( < 0.05) Enteritidis adherence to Cancer coli-2 (Caco-2) cells at 0.1 mg/ mL while MOS and FOS showed significant reduction at 1.0 mg/mL and 10.0 mg/mL, respectively. In terms of Enteritidis clearance, oligosaccharide-treated macrophages showed better Enteritidis clearance over time at 50 µg/mL for Small, Big and MOS, while FOS required a concentration of 500 µg/mL for a similar effect. This data highlights that oligosaccharides from PKC, particularly those of lower DP, were more effective than MOS and FOS at reducing Enteritidis adherence and enhancing Enteritidis clearance in a cell culture model.
肠炎血清型（血清型）肠炎沙门氏菌是一种食源性病原体，常与受污染的家禽产品有关。本研究评估了从棕榈仁粕（PKC）（棕榈油工业的一种副产品）中提取的低聚糖的抗黏附及细胞内清除能力，并将其与商业益生元低聚果糖（FOS）和甘露寡糖（MOS）在体外抗肠炎沙门氏菌方面的功效进行了比较。基于聚合度（DP），PKC低聚糖进一步分为“小分子”（DP≤6）和“大分子”（DP>6）组分。结果表明，小分子和大分子PKC组分在浓度为0.1mg/mL时就能降低（<0.05）肠炎沙门氏菌对人结肠腺癌细胞系-2（Caco-2）细胞的黏附，而MOS和FOS分别在浓度为1.0mg/mL和10.0mg/mL时才表现出显著降低。在肠炎沙门氏菌清除方面，经低聚糖处理的巨噬细胞在小分子、大分子和MOS浓度为50μg/mL时，随着时间推移对肠炎沙门氏菌的清除效果更好，而FOS需要500μg/mL的浓度才能达到类似效果。该数据表明，PKC中的低聚糖，尤其是那些聚合度较低的低聚糖，在细胞培养模型中比MOS和FOS在降低肠炎沙门氏菌黏附和增强肠炎沙门氏菌清除方面更有效。
