Department of Dermatology, Beijing Children' s Hospital, Capital Medical University, National Center for Children' s Health, Beijing, 100045, China.
Department of Dermatovenereology, Maanshan people' s hospital, Maanshan, 243000, China.
BMC Pediatr. 2020 Feb 19;20(1):78. doi: 10.1186/s12887-020-1974-9.
The term vascular anomalies include various vascular tumors and vascular malformations, among them infantile hemangiomas and capillary malformations are the most well-known associated diseases in early ages. Multiple drugs have been introduced for intervention, but susceptibility test in vitro were scarcely reported.
To evaluate the inhibition effect of different drugs by adenosine triphosphate sensitivity assay in vitro before the treatment of infantile hemangiomas and capillary malformations.
Specimens were selected from 5 cases of infantile hemangiomas and 11 cases of capillary malformations. Propranolol, rapamycin, sildenafil and itraconazole were tested for their growth inhibition effect by using the adenosine triphosphate sensitivity assay.
Propranolol demonstrated inhibitory effects on infantile hemangiomas cells. Rapamycin and itraconazole both showed inhibitory effects on infantile hemangiomas cells and capillary malformations cells. Sildenafil has no growth inhibitory effect on infantile hemangiomas cells or capillary malformations cells.
Adenosine triphosphate sensitivity assay is a sensitive and useful testing method before the management of vascular anomalies, and individualized medication suggestions for the choice of therapeutic drugs were offered based on the testing result and together with a comprehensive evaluation of each infant.
脉管性畸形包括各种血管肿瘤和血管畸形，其中婴幼儿血管瘤和毛细血管畸形是婴幼儿期最常见的相关疾病。已经引入了多种药物进行干预，但体外药敏试验报道甚少。
在治疗婴幼儿血管瘤和毛细血管畸形之前，通过三磷酸腺苷敏感性测定体外评估不同药物的抑制作用。
选取婴幼儿血管瘤 5 例，毛细血管畸形 11 例，用三磷酸腺苷敏感性测定法检测普萘洛尔、雷帕霉素、西地那非和伊曲康唑对其生长的抑制作用。
普萘洛尔对婴幼儿血管瘤细胞有抑制作用。雷帕霉素和伊曲康唑均对婴幼儿血管瘤细胞和毛细血管畸形细胞有抑制作用。西地那非对婴幼儿血管瘤细胞或毛细血管畸形细胞无生长抑制作用。
三磷酸腺苷敏感性测定是血管异常管理前一种敏感且有用的检测方法，根据检测结果和对每个患儿的综合评估，为选择治疗药物提供个体化的用药建议。
