Sen Research Group, Department of Biochemistry, Faculty of Arts and Science, Dumlupinar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey.
Department of Environmental Engineering, Faculty of Engineering, University of Igdir, Igdir, Turkey.
Sci Rep. 2020 Feb 19;10(1):2946. doi: 10.1038/s41598-020-59935-y.
In this study, we report a facile and effective production process of palladium nanoparticles supported on polypyrrole/reduced graphene oxide (rGO/Pd@PPy NPs). A novel electrochemical sensor was fabricated by incorporation of the prepared NPs onto glassy carbon electrode (GCE) for the simultaneous detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA). The electrodes modified with rGO/Pd@PPy NPs were well decorated on the GCE and exhibited superior catalytic activity and conductivity for the detection of these molecules with higher current and oxidation peak intensities. Simultaneous detection of these molecules was achieved due to the high selectivity and sensitivity of rGO/Pd@PPy NPs. For each biomolecule, well-separated voltammetric peaks were obtained at the modified electrode in cyclic voltammetry (CV) and differential pulse voltammetry (DPV) measurements. Additionally, the detection of these molecules was performed in blood serum samples with satisfying results. The detection limits and calibration curves for AA, DA, and UA were found to be 4.9 × 10, 5.6 × 10, 4.7 × 10 M (S/N = 3) and ranging from 1 × 10 to 1.5 × 10 M (in 0.1 M PBS, pH 3.0), respectively. Hereby, the fabricated rGO/Pd@PPy NPs can be used with high reproducibility, selectivity, and catalytic activity for the development of electrochemical applications for the simultaneous detection of these biomolecules.
在这项研究中，我们报告了一种简便有效的钯纳米粒子负载在聚吡咯/还原氧化石墨烯（rGO/Pd@PPy NPs）上的制备方法。通过将制备的纳米粒子掺入到玻碳电极（GCE）上，制备了一种新型电化学传感器，用于同时检测抗坏血酸（AA）、多巴胺（DA）和尿酸（UA）。rGO/Pd@PPy NPs 修饰的电极很好地修饰在 GCE 上，表现出更高的电流和氧化峰强度，对这些分子具有更高的催化活性和导电性。由于 rGO/Pd@PPy NPs 的高选择性和灵敏度，实现了这些分子的同时检测。对于每个生物分子，在循环伏安法（CV）和差分脉冲伏安法（DPV）测量中，在修饰电极上都获得了很好分离的伏安峰。此外，还在血清样品中进行了这些分子的检测，结果令人满意。AA、DA 和 UA 的检测限和校准曲线分别为 4.9×10、5.6×10 和 4.7×10 M（S/N=3）和 1×10 至 1.5×10 M（在 0.1 M PBS，pH 3.0）范围内。因此，所制备的 rGO/Pd@PPy NPs 可以具有高重现性、选择性和催化活性，用于开发电化学应用，同时检测这些生物分子。
