Department of Neurology, Shengli Oilfield Central Hospital, Dongying, Shandong, China.
Mol Genet Genomic Med. 2020 Apr;8(4):e1189. doi: 10.1002/mgg3.1189. Epub 2020 Feb 20.
Dysregulation of microRNAs (miRNAs) has been reported to be involved in the neuroinflammatory pathogenesis of PD. This study aimed to investigate the serum expression of microRNA-150 (miR-150) in Parkinson's disease (PD) patients and further uncover the regulatory effect of miR-150 on neuroinflammation.
Quantitative Real-Time PCR was used to measure the expression of miR-150. A receiver operating characteristic curve was applied to evaluate the diagnostic value of miR-150. The effect of miR-150 on neuroinflammation was analyzed by examining its correlation with proinflammatory cytokines and gain-of-function experiments in microglia treated with LPS.
Serum miR-150 expression was downregulated in PD patients compared with the healthy controls, and served as a candidate diagnostic biomarker for the screening of PD cases. Negative correlation was found between miR-150 levels and the levels of procytokines in PD patients. By the treatment of LPS, microglia BV2 cells had a reduced expression of miR-150, and the enhanced neuroinflammatory responses were inhibited by the overexpression of miR-150. AKT3 was verified as a target of miR-150 in BV2 cells.
All the data of this study revealed that the decreased serum miR-150 serves as a potential diagnostic biomarker. The methods to increase miR-150 expression may have a beneficial effect in PD via suppressing the neuroinflammation by targeting AKT3.
已有研究报道，微小 RNA（miRNA）的失调与 PD 的神经炎症发病机制有关。本研究旨在探讨帕金森病（PD）患者血清 miR-150 的表达情况，并进一步揭示 miR-150 对神经炎症的调控作用。
采用实时定量 PCR 检测 miR-150 的表达。应用受试者工作特征曲线评估 miR-150 的诊断价值。通过 LPS 处理的小胶质细胞中检测促炎细胞因子及其功能获得实验来分析 miR-150 对神经炎症的影响。
与健康对照组相比，PD 患者血清 miR-150 表达下调，可作为 PD 病例筛查的候选诊断生物标志物。PD 患者 miR-150 水平与前细胞因子水平呈负相关。经 LPS 处理后，BV2 细胞中 miR-150 表达减少，而过表达 miR-150 可抑制增强的神经炎症反应。AKT3 被验证为 BV2 细胞中 miR-150 的靶标。
本研究的所有数据表明，血清 miR-150 表达降低可作为一种潜在的诊断生物标志物。通过靶向 AKT3 增加 miR-150 的表达可能对 PD 具有有益的治疗作用，从而抑制神经炎症。
