长链非编码 RNA NEAT1/miR-185-5p/IGF2 轴调控结肠癌的侵袭和迁移。

LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer.

机构信息

Department of Gastrointestinal Surgery, Health Science Center, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China.

Shenzhen Second People's Hospital, Shenzhen, Guangdong, China.

出版信息

Mol Genet Genomic Med. 2020 Apr;8(4):e1125. doi: 10.1002/mgg3.1125. Epub 2020 Feb 20.

BACKGROUND

Long noncoding RNAs (lncRNA) are important in the growth and metastasis of colon cancer. The objective of this study was to describe the potential role of lncRNA NEAT1 in the progression of colon cancer.

METHODS

Quantitative real-time polymerase chain reaction was used for detecting NEAT1, miR-185-5p, and IGF2 in colon cancer cells and tissues. The potential diagnostic value of NEAT1 in colon cancer was analyzed with the receiver operating characteristic curve. Kaplan-Meier method was applied for evaluating the association between NEAT1 expression and the overall survival of osteosarcoma patients, whereas Transwell assay was introduced to examine the potential invasion and migration of colon cancer cells. In addition, the binding of NEAT1/IGF2 to miR-185-5p was confirmed by RNA pull-down and RNA-binding protein immunoprecipitation assays and dual-luciferase reporter gene assay. Finally, rescue experiments were conducted to confirm the role of NEAT1/miR-185-5p/IGF2 axis in colon cancer.

RESULTS

Colon cancer patients with low NEAT1 expression presented with longer overall survival than those with high expression. The migration and invasion of colon cancer cells were considerably promoted by overexpressed NEAT1. Both NEAT1 and IGF2 bound to miR-185-5p.

CONCLUSION

NEAT1 upregulate IGF2 expression through absorbing miR-185-5p to enhances the migration and invasion of colon cancer cells.

背景

长链非编码 RNA（lncRNA）在结肠癌的生长和转移中具有重要作用。本研究旨在描述 lncRNA NEAT1 在结肠癌进展中的潜在作用。

方法

采用实时定量聚合酶链反应检测结肠癌细胞和组织中的 NEAT1、miR-185-5p 和 IGF2。采用受试者工作特征曲线分析 NEAT1 在结肠癌中的潜在诊断价值。Kaplan-Meier 法评估 NEAT1 表达与骨肉瘤患者总生存期的关系，Transwell 检测分析结肠癌细胞的潜在侵袭和迁移能力。此外，通过 RNA 下拉和 RNA 结合蛋白免疫沉淀实验以及双荧光素酶报告基因实验证实了 NEAT1/IGF2 与 miR-185-5p 的结合。最后，通过 rescue 实验证实了 NEAT1/miR-185-5p/IGF2 轴在结肠癌中的作用。