Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Appl Microbiol Biotechnol. 2020 Apr;104(8):3627-3641. doi: 10.1007/s00253-020-10466-6. Epub 2020 Feb 20.
Plants associate with numerous microbes, but little is known about how microbiome components, especially fungi, adapt to specific plant compartments. The adaptability of microbial function to the plant compartment is also not clear especially for woody species. Here, we characterized the bacterial and fungal communities in root endosphere, stems, and rhizospheres of 33 Broussonetia papyrifera seedlings, based on amplification of 16S and ITS rRNA. Results showed that the α-diversity indexes of the bacterial community were significantly different in different plant compartments and they significantly increased from stem to root endosphere to the rhizosphere, whereas those of the fungal community were similar (p > 0.05). However, the result of constrained PCoA (CPCoA) and analysis of similarity (ANOSIM) showed that both bacterial and fungal compositions were significantly affected by plant compartments (p < 0.01). In detail, the operational taxonomic units (OTUs) distribution of the bacterial community was significantly different, but 249 of 252 fungal OTUs were shared in different plant compartments. Both the bacterial and fungal compositions were significantly influenced by plant compartments, based on the result on phyla, core OTUs, and indicator OTUs level. Further, 40 of 42 enriched KEGG pathways involving the bacteria also differed significantly among plant compartments (p < 0.01). This study provides an understanding of the influence of plant compartments on the microbiome and confirms that the disperse limitation of fungal OTUs across different plant compartments is smaller. This study sheds light on how the microbial community adapts to and thrives in different plant compartments.
植物与众多微生物共生，但对于微生物组成分（尤其是真菌）如何适应特定植物区室，我们知之甚少。微生物功能对植物区室的适应性也不清楚，尤其是对于木本物种。在这里，我们根据 16S 和 ITS rRNA 的扩增，对 33 株构树幼苗的根内圈、茎和根际中的细菌和真菌群落进行了特征描述。结果表明，不同植物区室的细菌群落 α 多样性指数存在显著差异，从茎到根内圈再到根际呈显著增加趋势，而真菌群落则相似（p>0.05）。然而，受限主坐标分析（CPCoA）和相似性分析（ANOSIM）的结果表明，细菌和真菌组成均受到植物区室的显著影响（p<0.01）。具体而言，细菌群落的操作分类单元（OTUs）分布存在显著差异，但在不同植物区室中，有 249 个真菌 OTUs 是共有的。基于门、核心 OTUs 和指示 OTUs 水平的结果，细菌和真菌组成均受到植物区室的显著影响。此外，涉及细菌的 42 个富集 KEGG 途径中有 40 个在植物区室之间也存在显著差异（p<0.01）。本研究提供了对植物区室对微生物组影响的理解，并证实了真菌 OTUs 在不同植物区室之间的分散限制较小。本研究揭示了微生物群落如何适应和在不同植物区室中繁衍生息。
