Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Appl Environ Microbiol. 2020 Sep 1;86(18). doi: 10.1128/AEM.00464-20.
The genetic basis of plant local adaptation has been extensively studied, yet the interplay between local adaptation, plant genetic divergence, and the microbial community remains unclear. Our study used the restriction-site associated DNA sequencing (RAD-seq) approach to explore genetic divergence in and used internal transcribed spacers (ITS) to characterize fungal community. RAD-seq results show that individuals could be divided into three genotypes; this genotyping result was consistent with the classification of climate type at the sample site. Most of the 101 highly differentiated genes were related to stress resistance and the microbiome. Moreover, β-diversity results indicated that genetic divergence had a significant effect on fungal community across all compartments (0.01). At genus and operational taxonomic unit (OTU) level, , , OTU81578 (), and OTU1665209 () were found to be the major OTUs that contribute to differences in fungal community. The properties of cooccurrence networks vary greatly among three genotypes. The results of redundancy analysis (RDA) indicated that -associated fungal community was significantly related to its local adaptability. Our findings suggest that genetic divergence of is closely related to local adaptation, with significant effects on the associated fungal community, which in turn would enhance host local adaptability. This improves present understanding about the coevolution of microbial communities and the host plant. The coevolution of plants with the associated fungal community and its effect on plant adaptability are not clear, especially for native trees. This study focuses on the genetic basis of local adaptation in plants and the effect of genetic divergence of on the associated fungal community. We identified genes related to the microbiome that are important for local adaptation of the host. Our results show that genetic divergence in significantly affects the fungal community, which has a close connection with local adaptation. This helps us to understand the relationship between local adaptation, genetic divergence, and associated fungal communities. This study highlights the effect of plant genetic divergence on associated fungal community for native trees and establishes a close connection between this effect and local adaptability in the host. In addition, these observations lay a foundation for the research of coevolution of plants and their symbiotic microbiome through genome-wide association study (GWAS).
植物局部适应的遗传基础已得到广泛研究，但局部适应、植物遗传分化和微生物群落之间的相互作用仍不清楚。我们的研究使用了限制性位点相关 DNA 测序（RAD-seq）方法来探索 中的遗传分化，并使用内部转录间隔区（ITS）来描述真菌群落。RAD-seq 结果表明， 个体可分为三种基因型；这种基因分型结果与样本地点的气候类型分类一致。101 个高度分化的基因大多数与抗逆性和微生物组有关。此外，β多样性结果表明，遗传分化对所有隔室（0.01）的真菌群落有显著影响。在属和操作分类单元（OTU）水平上， 、 、OTU81578（）和 OTU1665209（）被发现是导致真菌群落差异的主要 OTU。三种基因型的共生网络性质差异很大。冗余分析（RDA）的结果表明， 相关的真菌群落与其局部适应性显著相关。我们的研究结果表明， 的遗传分化与其局部适应性密切相关，对相关真菌群落有显著影响，进而增强宿主的局部适应性。这提高了我们对微生物群落与宿主植物共同进化的理解。植物与相关真菌群落的共同进化及其对植物适应性的影响尚不清楚，特别是对于本地树木。本研究关注植物局部适应的遗传基础以及 遗传分化对相关真菌群落的影响。我们确定了与微生物组相关的对宿主局部适应很重要的基因。我们的研究结果表明， 中遗传分化的显著影响真菌群落，这与局部适应密切相关。这有助于我们理解局部适应、遗传分化和相关真菌群落之间的关系。本研究强调了植物遗传分化对本地树木相关真菌群落的影响，并建立了这种影响与宿主局部适应性之间的紧密联系。此外，这些观察结果为通过全基因组关联研究（GWAS）研究植物与其共生微生物组的共同进化奠定了基础。
