Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente, Departamento de Microbiologia, Universidade Federal de Viçosa, Av. P.H. Rolfs s/n, Centro, Viçosa, Minas Gerais, Brazil.
Instituto Federal de Educação, Ciência e Tecnologia do Ceará - IFCE - campus Camocim, Camocim, Ceará, Brazil.
Braz J Microbiol. 2020 Sep;51(3):1159-1168. doi: 10.1007/s42770-020-00244-x. Epub 2020 Feb 20.
We studied petroleum biodegradation by biostimulation by using water in oil in water (W/O/W) double emulsions. These emulsions were developed using seawater, canola oil, surfactants, and mineral salts as sources of NPK. The emulsions were used in the simulation of hydrocarbon bioremediation in oligotrophic sea water. Hydrocarbon biodegradation was evaluated by CO emissions from microcosms. We also evaluated the release of inorganic nutrients and the stability of the emulsion's droplets. The double emulsions improved CO emission from the microcosms, suggesting the increase in the hydrocarbon biodegradation. Mineral nutrients were gradually released from the emulsions supporting the hydrocarbon biodegradation. This was attributed to the formation of different diameters of droplets and therefore, varying stabilities of the droplets. Addition of the selected hydrocarbonoclastic isolates simulating bioaugmentation improved the hydrocarbon biodegradation. We conclude that the nutrient-rich W/O/W emulsion developed in this study is an effective biostimulation agent for bioremediation in oligotrophic aquatic environments.
我们通过使用油包水包水（W/O/W）双重乳液进行生物刺激来研究石油生物降解。这些乳液是使用海水、菜籽油、表面活性剂和矿物盐作为 NPK 来源开发的。这些乳液用于模拟贫营养海水中的烃类生物修复。通过微宇宙中的 CO 排放来评估烃类生物降解。我们还评估了无机养分的释放和乳液液滴的稳定性。双重乳液增加了微宇宙中的 CO 排放，表明烃类生物降解增加。矿物养分逐渐从乳液中释放出来，支持烃类的生物降解。这归因于不同直径液滴的形成，因此，液滴的稳定性也不同。添加模拟生物增强的选定的烃类降解菌提高了烃类生物降解。我们的结论是，本研究中开发的富含营养的 W/O/W 乳液是贫营养水生环境生物修复的有效生物刺激剂。
