Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology. Faculty of Chemistry. Universidade de Santiago de Compostela, Avenida Das Ciencias, S/n, 15782, Santiago de Compostela, Spain.
Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology. Faculty of Chemistry. Universidade de Santiago de Compostela, Avenida Das Ciencias, S/n, 15782, Santiago de Compostela, Spain.
Anal Chim Acta. 2020 Mar 22;1103:183-191. doi: 10.1016/j.aca.2019.12.060. Epub 2019 Dec 24.
Possibilities of room temperature spectrometry based on Mn-doped ZnS quantum dots coated with a molecularly imprinted polymer based nanosensor have been explored for the sensitive and selective determination of aflatoxins. Synthesized polymeric nanoparticles exhibit intense room temperature phosphorescence (total decay time of 0.004 s) and aflatoxins quench the room temperature phosphorescence when interact with the recognition cavities of the molecularly imprinted polymer attached to the phosphorescent quantum dots. Room temperature phosphorescence was recorded by scanning from 520 nm to 720 nm (maximum peak intensity at 594 nm) after excitation at 290 nm. The prepared imprinted material was found to have higher adsorption capacity than those based non-imprinted quantum dots, demonstrating high adsorption uptake for aflatoxins. In addition, selectivity studies have demonstrated that the material offers a specific recognition for aflatoxins. Room temperature phosphorescence quenching by aflatoxins was found to be linear within the 2-20 μg L range, and a limit of detection of 3.56 μg kg was obtained. This value was lower than the maximum acceptable/residual level (aflatoxins in feeds) published by the European Commission. The results indicate a simple room temperature phosphorescence nanosensor for aflatoxins detection in fish feed as a versatile tool having excellent sensitivity and selectivity.
基于 Mn 掺杂的 ZnS 量子点的室温光谱法的可能性已经被探索用于灵敏和选择性地测定黄曲霉毒素。合成的聚合物纳米粒子表现出强烈的室温磷光（总衰减时间为 0.004s），并且当黄曲霉毒素与附着在磷光量子点上的分子印迹聚合物的识别腔相互作用时，会猝灭室温磷光。在激发波长为 290nm 后，通过从 520nm 扫描到 720nm（最大峰值强度在 594nm）记录室温磷光。发现制备的印迹材料具有比非印迹量子点更高的吸附容量，表明对黄曲霉毒素具有高吸附能力。此外，选择性研究表明，该材料对黄曲霉毒素具有特异性识别。黄曲霉毒素对室温磷光的猝灭在 2-20μg L 范围内呈线性，检测限为 3.56μg kg。该值低于欧盟委员会公布的最大允许/残留水平（饲料中的黄曲霉毒素）。结果表明，作为一种具有优异灵敏度和选择性的通用工具，该方法提供了一种用于鱼饲料中黄曲霉毒素检测的简单室温磷光纳米传感器。
