基于锰掺杂硫化锌量子点-分子印迹聚合物荧光探针的非乳制品饮料中黄曲霉毒素的筛选
Aflatoxins screening in non-dairy beverages by Mn-doped ZnS quantum dots - Molecularly imprinted polymer fluorescent probe.
机构信息
Laboratory of Hetero-Organic Compounds and Nanostructured Materials (LR18ES11), Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia; Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 15782 Santiago de Compostela, Spain.
Laboratory of Hetero-Organic Compounds and Nanostructured Materials (LR18ES11), Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
出版信息
Talanta. 2019 Jul 1;199:65-71. doi: 10.1016/j.talanta.2019.02.057. Epub 2019 Feb 14.
Synthesized molecularly imprinted polymer (MIP) materials have been anchored on the surface of PEG-Mn-doped ZnS quantum dots (QDs) to develop a fluorescence probe for aflatoxins (AFs) recognition/determination in non-dairy beverages. MIP synthesis used 5,7-dimethoxycoumarin (DMC) as a dummy template molecule, and methacrylic acid (MAA) as a functional monomer. Under optimized conditions (1.25 mL of 5 mg L MIP-PEG-ZnS QDs solution, pH 5.0, and 12 min delay time before scanning), the prepared MIP-QDs composite was found to offer high affinity and selectivity for AFs (AFB1, AFB2, AFG1 and AFG2). A fast fluorimetric screening method (total AFs assessment) was therefore feasible. The limits of detection (LOD) and quantification (LOQ) were 0.016 and 0.053 mg L, respectively. Analytical recoveries (inter- and intra-day assays) were from 99 ± 4% to 107 ± 5%, with RSD (intra-day assay) lower than 13, and RSD (inter-day) lower than 7%. The optimized method was applied for total AFs assessment in several non-dairy beverage samples.
已将合成的分子印迹聚合物 (MIP) 材料固定在 PEG-Mn 掺杂的 ZnS 量子点 (QDs) 的表面上，以开发用于非乳制品饮料中黄曲霉毒素 (AFs) 识别/测定的荧光探针。MIP 合成使用 5,7-二甲氧基香豆素 (DMC) 作为虚拟模板分子，甲基丙烯酸 (MAA) 作为功能单体。在优化条件下（1.25 mL 5 mg L MIP-PEG-ZnS QDs 溶液，pH 值 5.0，扫描前延迟 12 分钟），发现制备的 MIP-QDs 复合材料对 AFs（AFB1、AFB2、AFG1 和 AFG2）具有高亲和力和选择性。因此，建立了快速荧光筛选方法（总 AFs 评估）。检测限（LOD）和定量限（LOQ）分别为 0.016 和 0.053 mg L。分析回收率（日内和日间测定）为 99 ± 4%至 107 ± 5%，日内测定的 RSD 低于 13%，日间测定的 RSD 低于 7%。优化后的方法用于几种非乳制品饮料样品中总 AFs 的评估。
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