Bratlid D
Acta Pathol Microbiol Scand C. 1977 Feb;85(1):17-20. doi: 10.1111/j.1699-0463.1977.tb03605.x.
A new procedure for spectrophotometric determination of bacteriolytic activity in biological fluids is described. The method uses Micrococcus lysodeikticus cells as substrate. By inactivation of the sample through pre-incubation in ice-water, a large number of samples can be prepared and analysed simultaneously. The necessity of performing the whole analysis in the photometer for one sample at the time is thus eliminated. By measuring the increase in transmission at 570 nm after incubation of the samples at 37 degrees C a relatively long reaction time and wide concentration range is obtained. This makes the results quite precise and reproducible. The method has been used to determine the bacteriolytic activity in serum of healthy adults. Men have significantly higher levels than women.
本文描述了一种用于分光光度法测定生物流体中溶菌活性的新方法。该方法使用溶壁微球菌细胞作为底物。通过在冰水浴中预孵育使样品失活，可以同时制备和分析大量样品。这样就消除了一次仅在光度计中对一个样品进行整个分析的必要性。通过测量样品在37℃孵育后570nm处透光率的增加，可获得相对较长的反应时间和较宽的浓度范围。这使得结果相当精确且可重复。该方法已用于测定健康成年人血清中的溶菌活性。男性的水平明显高于女性。
