Huot Camille, Clerissi Camille, Gourbal Benjamin, Galinier Richard, Duval David, Toulza Eve
IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Perpignan, France.
Front Microbiol. 2020 Jan 31;10:3092. doi: 10.3389/fmicb.2019.03092. eCollection 2019.
Planorbidae snails are the intermediate host for the trematode parasite of the genus, which is responsible for schistosomiasis, a disease that affects both humans and cattle. The microbiota for has already been described as having an effect on host/parasite interactions, specifically through immunological interactions. Here, we sought to characterize the microbiota composition of seven Planorbidae species and strains. Individual snail microbiota was determined using 16S ribosomal DNA amplicon sequencing. The bacterial composition was highly specific to the host strain with limited interindividual variation. In addition, it displayed complete congruence with host phylogeny, revealing a phylosymbiosis pattern. These results were confirmed in a common garden, suggesting that the host highly constrains microbial composition. This study presents the first comparison of bacterial communities between several intermediate snail hosts of parasites, paving the way for further studies on the understanding of this tripartite interaction.
扁卷螺科蜗牛是该属吸虫寄生虫的中间宿主，这种寄生虫会导致血吸虫病，一种影响人类和牛的疾病。已经有研究表明，扁卷螺科蜗牛的微生物群会对宿主/寄生虫相互作用产生影响，特别是通过免疫相互作用。在此，我们试图对7种扁卷螺科物种和菌株的微生物群组成进行表征。使用16S核糖体DNA扩增子测序来确定单个蜗牛的微生物群。细菌组成对宿主菌株具有高度特异性，个体间差异有限。此外，它与宿主系统发育完全一致，揭示了一种系统共生模式。这些结果在一个共同环境中得到了证实，表明宿主对微生物组成有高度的限制作用。这项研究首次比较了几种寄生虫中间蜗牛宿主之间的细菌群落，为进一步研究这种三方相互作用奠定了基础。
