Portet Anaïs, Toulza Eve, Lokmer Ana, Huot Camille, Duval David, Galinier Richard, Gourbal Benjamin
IHPE, University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, 66860 Perpignan, France.
Laboratory of Eco-Anthropology UMR 7206 CNRS-MNHN-Paris 7, 75005 Paris, France.
Microorganisms. 2021 May 18;9(5):1084. doi: 10.3390/microorganisms9051084.
Host-parasite interaction can result in a strong alteration of the host-associated microbiota. This dysbiosis can affect the fitness of the host; can modify pathogen interaction and the outcome of diseases. is the snail intermediate host of the trematode , the agent of human schistosomiasis, causing hundreds of thousands of deaths every year. Here, we present the first study of the snail bacterial microbiota in response to infection. We examined the interplay between and host microbiota. Snails were infected and the microbiota composition was analysed by 16S rDNA amplicon sequencing approach. We demonstrated that the microbial composition of water did not affect the microbiota composition. Then, we characterised the bacterial microbiota at the individual scale in both naive and infected snails. Sympatric and allopatric strains of parasites were used for infections and re-infections to analyse the modification or dysbiosis of snail microbiota in different host-parasite co-evolutionary contexts. Concomitantly, using RNAseq, we investigated the link between bacterial microbiota dysbiosis and snail anti-microbial peptide immune response. This work paves the way for a better understanding of snail/schistosome interaction and should have critical consequences in terms of snail control strategies for fighting schistosomiasis disease in the field.
宿主 - 寄生虫相互作用可导致宿主相关微生物群的强烈改变。这种生态失调会影响宿主的健康状况，能改变病原体相互作用及疾病的转归。钉螺是吸虫的中间宿主，吸虫是人类血吸虫病的病原体，每年导致成千上万的人死亡。在此，我们首次开展了关于钉螺细菌微生物群对吸虫感染反应的研究。我们研究了吸虫与宿主微生物群之间的相互作用。将钉螺感染后，采用16S rDNA扩增子测序方法分析微生物群组成。我们证明水的微生物组成不会影响钉螺微生物群的组成。然后，我们在个体水平上对未感染和已感染钉螺的细菌微生物群进行了表征。使用同域和异域寄生虫菌株进行感染和再感染，以分析在不同宿主 - 寄生虫共同进化背景下钉螺微生物群的改变或失调。同时，利用RNA测序技术，我们研究了细菌微生物群失调与钉螺抗菌肽免疫反应之间的联系。这项工作为更好地理解钉螺/血吸虫相互作用铺平了道路，并且在实地控制血吸虫病的钉螺控制策略方面应该会产生关键影响。
