Gong Wenbing, Xie Chunliang, Zhou Yingjun, Zhu Zuohua, Wang Yahui, Peng Yuande
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China.
Front Microbiol. 2020 Jan 31;10:3129. doi: 10.3389/fmicb.2019.03129. eCollection 2019.
has attracted tremendous interest owing to its compelling health-promoting properties. However, breeding of elite cultivars of is hindered by the lack of a genetic and molecular toolbox. Here, we performed resequencing analysis of 127 F single-spore isolates and constructed the first high-resolution genetic map of . With the use of recombination bins as markers, an ultradense genetic map consisting of 1,174 bins (including 37,082 single-nucleotide polymorphisms) was generated. This newly developed genetic map covered 1,096.5 cM, with an average bin spacing of 0.95 cM. High collinearity between genetic map and genome assembly was revealed by aligning scaffolds to this genetic map using bin markers as anchors. The application of this newly developed genetic map in quantitative trait locus (QTL) mapping was also elucidated, and four QTLs for monokaryon growth were recovered. One QTL, , which contributes 12.1% of growth variations, was located near the mating type A (MAT-A) loci. Overall, this newly constructed high-resolution genetic map (or bin map) could be used as reference in future genetic, genomic, and breeding studies on .
由于其具有令人信服的促进健康特性，已引起了极大的关注。然而，缺乏遗传和分子工具阻碍了优良品种的培育。在此，我们对127个F单孢分离株进行了重测序分析，并构建了首个高分辨率的遗传图谱。以重组区间作为标记，生成了一个由1174个区间（包括37082个单核苷酸多态性）组成的超密集遗传图谱。这个新开发的遗传图谱覆盖了1096.5厘摩，平均区间间距为0.95厘摩。通过使用区间标记作为锚定物将支架与该遗传图谱进行比对，揭示了遗传图谱与基因组组装之间的高度共线性。还阐明了这个新开发的遗传图谱在数量性状位点（QTL）定位中的应用，并鉴定出了四个控制单核体生长的QTL。其中一个名为 的QTL，对生长变异的贡献率为12.1%，位于A交配型（MAT-A）位点附近。总体而言，这个新构建的高分辨率遗传图谱（或区间图谱）可作为未来关于 遗传、基因组和育种研究的参考。
