Gong Wen-Bing, Li Lei, Zhou Yan, Bian Yinbing, Kwan Hoi-Shan, Cheung Man-Kit, Xiao Yang
Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, People's Republic of China; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, People's Republic of China.
School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, People's Republic of China.
Int J Med Mushrooms. 2018;20(5):451-458. doi: 10.1615/IntJMedMushrooms.2018026236.
Increasing yield is a principal goal when breeding Lentinus edodes. The detection of quantitative trait loci (QTLs) underlying yield and its related traits, precocity and the number of fruiting bodies (NFs), is important in order to breed high-yield cultivars. Using composite interval mapping (CIM), we mapped a total of 25 QTLs responsible for precocity, NFs, and yield in 2 segregating populations of L. edodes. QTLs for the 3 traits were mapped on 5 different linkage groups, contributing 5.9% to 15.4% of the phenotypic variation. Colocated QTLs were also found underlying multiple traits, implying the presence of a genic pleiotropic effect or tightly linked genes. This is, to our knowledge, the first report of the genetic dissection of precocity, NFs, and yield using QTL mapping in L. edodes. Findings of this study will facilitate marker-assisted breeding of high-yield cultivars of L. edodes.
提高产量是香菇育种的主要目标。检测产量及其相关性状（早熟性和子实体数量）的数量性状位点（QTL）对于培育高产香菇品种很重要。利用复合区间作图法（CIM），我们在香菇的2个分离群体中总共定位了25个与早熟性、子实体数量和产量相关的QTL。这3个性状的QTL被定位在5个不同的连锁群上，对表型变异的贡献率为5.9%至15.4%。还发现多个性状存在共定位QTL，这意味着存在基因多效性效应或紧密连锁的基因。据我们所知，这是首次利用QTL定位对香菇早熟性、子实体数量和产量进行遗传剖析的报道。本研究结果将有助于香菇高产品种的分子标记辅助育种。
