Maldonado-Taipe Nathaly, Barbier Federico, Schmid Karl, Jung Christian, Emrani Nazgol
Plant Breeding Institute, Christian-Albrechts-University of Kiel, Kiel, Germany.
Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Stuttgart, Germany.
Front Plant Sci. 2022 Jun 9;13:916067. doi: 10.3389/fpls.2022.916067. eCollection 2022.
Quinoa is a pseudocereal originating from the Andean regions. Despite quinoa's long cultivation history, genetic analysis of this crop is still in its infancy. We aimed to localize quantitative trait loci (QTL) contributing to the phenotypic variation of agronomically important traits. We crossed the Chilean accession PI-614889 and the Peruvian accession CHEN-109, which depicted significant differences in days to flowering, days to maturity, plant height, panicle length, and thousand kernel weight (TKW), saponin content, and mildew susceptibility. We observed sizeable phenotypic variation across F plants and F families grown in the greenhouse and the field, respectively. We used Skim-seq to genotype the F population and constructed a high-density genetic map with 133,923 single nucleotide polymorphism (SNPs). Fifteen QTL were found for ten traits. Two significant QTL, common in F and F generations, depicted pleiotropy for days to flowering, plant height, and TKW. The pleiotropic QTL harbored several putative candidate genes involved in photoperiod response and flowering time regulation. This study presents the first high-density genetic map of quinoa that incorporates QTL for several important agronomical traits. The pleiotropic loci can facilitate marker-assisted selection in quinoa breeding programs.
藜麦是一种源自安第斯地区的假谷物。尽管藜麦有着悠久的种植历史，但对这种作物的遗传分析仍处于起步阶段。我们旨在定位影响重要农艺性状表型变异的数量性状基因座（QTL）。我们将智利种质PI - 614889和秘鲁种质CHEN - 109进行杂交，这两个种质在开花天数、成熟天数、株高、穗长、千粒重（TKW）、皂苷含量和对白粉病的易感性方面存在显著差异。我们分别在温室和田间种植的F植株和F家系中观察到了相当大的表型变异。我们使用Skim - seq对F群体进行基因分型，并构建了一个包含133,923个单核苷酸多态性（SNP）的高密度遗传图谱。针对十个性状发现了15个QTL。在F和F代中共同存在的两个显著QTL，对开花天数、株高和TKW表现出多效性。该多效性QTL包含几个参与光周期反应和开花时间调控的假定候选基因。本研究展示了首个藜麦高密度遗传图谱，该图谱纳入了多个重要农艺性状的QTL。这些多效性位点有助于藜麦育种计划中的标记辅助选择。
