Department of Hematology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang, China.
Department of Clinical Laboratory, Biobank, Harbin Medical University Cancer Hospital, Harbin 150040, Heilongjiang, China.
Biosci Rep. 2020 Feb 28;40(2). doi: 10.1042/BSR20194202.
Wilms tumor is the most common pediatric malignancy in the kidney. The miR34b/c is a downstream target gene of the transcription factor p53. The important role of TP53 mutations, the methylation of miR34b/c, and the interaction between these two molecules in tumorigenesis have been well documented. Due to the biological connection between p53 and miR34b/c, in the present study, we investigated the association between polymorphisms in these two molecules and Wilms tumor susceptibility through genotyping two important functional polymorphisms (miR34b/c rs4938723 T>C and TP53 rs1042522 C>G) in 183 cases and 603 controls. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) derived from the logistic regression analysis were used to assess the correlation of miR34b/c rs4938723 and TP53 rs1042522 polymorphisms with Wilms tumor risk. Our results indicated that the association of miR34b/c rs4938723 and TP53 rs1042522 polymorphisms with Wilms tumor susceptibility was not statistically significant. Stratified analysis by age, gender, and clinical stage, as well as combined effect analysis were also performed, yet, no significant association was found. In conclusion, our study indicated a lack of association between the two selected polymorphisms and Wilms tumor susceptibility. Our findings need to be verified in studies with larger sample size in the future.
威尔姆斯瘤是儿童肾脏最常见的恶性肿瘤。miR34b/c 是转录因子 p53 的下游靶基因。TP53 突变、miR34b/c 的甲基化以及这两个分子之间的相互作用在肿瘤发生中的重要作用已得到充分证实。由于 p53 和 miR34b/c 之间存在生物学联系，在本研究中，我们通过对这两个重要的功能性多态性（miR34b/c rs4938723 T>C 和 TP53 rs1042522 C>G）进行基因分型，研究了这两个分子的多态性与 Wilms 肿瘤易感性之间的关系。我们使用逻辑回归分析得出的调整后的比值比（OR）和 95％置信区间（CI）来评估 miR34b/c rs4938723 和 TP53 rs1042522 多态性与 Wilms 肿瘤风险之间的相关性。我们的结果表明，miR34b/c rs4938723 和 TP53 rs1042522 多态性与 Wilms 肿瘤易感性之间的关联没有统计学意义。我们还进行了年龄、性别和临床分期的分层分析以及合并效应分析，但未发现显著相关性。总之，我们的研究表明，这两个选定的多态性与 Wilms 肿瘤易感性之间没有关联。我们的发现需要在未来更大样本量的研究中进行验证。
